Carrier System of Chemical Drugs and Isotope from Gelatin Electrospun Nanofibrous Membranes
Carrier System of Chemical Drugs and Isotope from Gelatin Electrospun Nanofibrous Membranes
复制标题
明胶电纺纳米纤维膜化学药物和同位素的载体系统
DOI：
10.1021/bm100505j
复制
发表时间：
2010-08-01
影响因子：
6.2
通讯作者：
Han, Charles C.
中科院分区：
文献类型：
--
作者：
Nie, Huarong;Li, Junxing;Han, Charles C.
材料和方法。GE A型聚合物（约220 Bloom，粘度4.8 mPa· s）通过酸性工艺从猪皮中提取，购自三和盛明胶（中国温州）。YCl 3、双蒸水和乙醇从Beijing Chem.（中国北京）。N-羟基琥珀酰亚胺（NHS）获自Sigma。1-乙基-3-（3-二甲基氨基丙基）碳二亚胺（EDC）粉末和二亚乙基三胺五乙酸二酐（DTPA）购自Fluka。头孢拉定（CE）购自哈尔滨制药总厂（中国哈尔滨）。所有材料不经进一步纯化即使用。GE膜的制造。将15%（w/v）的GE溶液在体积比为9/1的水和乙醇的混合溶剂中进行静电纺丝，制备了GE纳米纤维。所有电纺丝溶液、纺丝液和环境的温度都控制在40 ℃。将纳米纤维收集在目标鼓上，该目标鼓放置在距注射器尖端15 cm的距离处（内径0.3mm）。通过高压电源向收集靶施加22 kV的电压，溶液的流速为50 μL/min。将纳米纤维在室温下真空干燥24 h以除去残留的溶剂。
Materials and Methods. Polymers of GE type A (approximately 220 Bloom, viscosity 4.8 mPa· s), extracted from porcine skin by acidic process, were purchased from Sanhesheng Gelatin (Wenzhou, China). YCl3, double-distilled water, and ethanol were obtained from Beijing Chem.(Beijing, China). N-Hydroxysuccinimide (NHS) was obtained from Sigma. 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) powder and diethylenetriaminepentaacetic acid dianhydride (DTPA) were purchased from Fluka. Cefradine (CE) was obtained from the General Pharmaceutical Factory of Harbin (Harbin, China). All materials were used without further purification. Fabrication of GE Membranes. GE nanofibers were prepared by electrospinning a 15%(w/v) GE solution in the blend solvents of water and ethanol, where the volume ratio is 9/1. The temperatures of all electrospinning solutions, spinneret, and the environment were controlled at 40 (3 C. Nanofibers were collected on a target drum that was placed at a distance of 15 cm from the syringe tip (inner diameter 0.3 mm). A voltage of 22 kV was applied to the collecting target by a high voltage power supply, and the flow rate of the solution was 50 μL/min. The nanofibers were dried in a vacuum for 24 h at room temperature to remove the remaining solvent.