Maternal Nicotine Exposure Induces Epithelial-Mesenchymal Transition in Rat Offspring Lungs
Maternal Nicotine Exposure Induces Epithelial-Mesenchymal Transition in Rat Offspring Lungs
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DOI：
10.1159/000437012
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发表时间：
2015-01-01
期刊：
影响因子：
2.5
通讯作者：
Huang, Liang-Ti
中科院分区：
文献类型：
--
作者：
Chen, Chung-Ming;Chou, Hsiu-Chu;Huang, Liang-Ti
背景：母体接触尼古丁会导致大鼠后代肺损伤和纤维化。肺损伤后的上皮 - 间质转化（EMT）是上皮细胞介导组织修复的一个过程。
目的：确定母体接触尼古丁对新生大鼠肺上皮 - 间质转化的影响。
方法：使用皮下渗透微泵给予怀孕的斯普拉格 - 道利大鼠尼古丁，在妊娠第7 - 21天或从妊娠第7天到产后第14天，剂量为6mg/kg/天。对照组给予等体积的生理盐水。
结果：在产后第7天，产前和产后接受尼古丁处理的母鼠所生大鼠核染色中8 - 羟基 - 2'- 脱氧鸟苷阳性细胞百分比显著升高，E - 钙黏蛋白表达显著降低，N - 钙黏蛋白表达显著升高。这些上皮 - 间质转化的特征与产后第21天α - 平滑肌肌动蛋白（SMA）表达显著增加有关。在产后第21天，产前和产后接受尼古丁处理的母鼠所生大鼠α - SMA表达和总胶原蛋白显著高于产前接受生理盐水和尼古丁处理的母鼠所生大鼠。在产后第7天和第21天，产前和产后接受尼古丁处理的母鼠所生大鼠表达成纤维细胞特异性蛋白1和波形蛋白的细胞数量多于产前接受生理盐水和尼古丁处理的母鼠所生大鼠。
结论：妊娠期和哺乳期母体接触尼古丁会诱导大鼠后代上皮 - 间质转化，并导致肺纤维化。（C）2015年巴塞尔S. 卡尔格股份公司
Background: Maternal nicotine exposure induces lung injuries and fibrosis in rat offspring. Epithelial-mesenchymal transition (EMT) following lung injury is a process in which epithelial cells mediate tissue repair. Objective: To determine the effects of maternal nicotine exposure on EMT in neonatal rat lungs. Methods: Nicotine was administered to pregnant Sprague-Dawley rats using a subcutaneous osmotic minipump that delivered a dose of 6 mg/kg/day on gestational days 7-21 or from gestational day 7 to postnatal day 14. A control group received an equal volume of saline. Results: The percentage of 8-hydroxy-2'-deoxyguanosine-positive cells in nuclear staining was significantly higher, the E-cadherin protein expression was significantly lower, and the N-cadherin protein expression was significantly higher in rats born to prenatal and postnatal nicotine-treated dams than in those born to prenatal saline-and nicotine-treated dams on postnatal day 7. These characteristics of EMT were associated with a significant increase in a-smooth muscle actin (SMA) expression on postnatal day 21. Rats born to prenatal and postnatal nicotine-treated dams showed significantly higher alpha-SMA expression and total collagen than those born to prenatal saline- and nicotine-treated dams on postnatal day 21. The number of cells expressing fibroblast-specific protein 1 and vimentin was higher in rats born to prenatal and postnatal nicotine-treated dams than in those born to prenatal saline- and nicotine-treated dams on postnatal days 7 and 21. Conclusions: Maternal nicotine exposure during gestation and lactation induces EMT and contributes to lung fibrosis in rat offspring. (C) 2015 S. Karger AG, Basel