Quantitative analysis and molecular species fingerprinting of triacylglyceride molecular species directly from lipid extracts of biological samples by electrospray ionization tandem mass spectrometry
Quantitative analysis and molecular species fingerprinting of triacylglyceride molecular species directly from lipid extracts of biological samples by electrospray ionization tandem mass spectrometry
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DOI：
10.1006/abio.2001.5178
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发表时间：
2001-08-01
影响因子：
2.9
通讯作者：
Gross, RW
中科院分区：
文献类型：
--
作者：
Han, XL;Gross, RW
本文描述了一种快速、简便、可靠的方法，用于直接从生物样品的氯仿提取物中定量分析和分子种类指纹图谱。先前通过正离子电喷雾电离质谱法（ESI/MS）进行直接TAG定量的尝试受到胆碱甘油磷脂重叠峰的干扰，需要在ESI/MS分析前对脂质提取物进行色谱分离。通过利用胆碱甘油磷脂中磷酸胆碱的快速损失，结合对单个脂肪酸的中性损失扫描，对ESI质谱中的重叠峰进行去卷积，直接从生物样品的氯仿提取物中生成单个TAG分子种类的详细分子种类指纹。该方法易于从氯仿提取物中检测到低至0.1 pmol的各TAG分子种类，并且在1000倍动态范围内呈线性。各个TAG分子种类对ESI/MS/MS分析的灵敏度与不饱和指数相关，与TAG的总脂肪链长度呈负相关。开发了一种识别灵敏度因子的算法，从而允许直接从生物样品的氯仿提取物中快速定量TAG分子种类并对其进行分子种类指纹分析。(C)北京：科学出版社.
Herein we describe a rapid, simple, and reliable method for the quantitative analysis and molecular species fingerprinting of triacylglycerides (TAG) directly from chloroform extracts of biological samples. Previous attempts at direct TAG quantitation by positive-ion electrospray ionization mass spectrometry (ESI/MS) were confounded by the presence of overlapping peaks from choline glycerophospholipids requiring chromatographic separation of lipid extracts prior to ESI/MS analyses. By exploiting the rapid loss of phosphocholine from choline glycerophospholipids, in conjunction with neutral-loss scanning for individual fatty acids, overlapping peaks in the ESI mass spectrum were deconvoluted generating a detailed molecular species fingerprint of individual TAG molecular species directly from chloroform extracts of biological samples. This method readily detects as little as 0.1 pmol of each TAG molecular species from chloroform extracts and is linear over a 1000-fold dynamic range. The sensitivity of individual TAG molecular species to ESI/MS/MS analyses correlated with the unsaturation index and inversely correlated with total aliphatic chain length of TAG. An algorithm was developed which identifies sensitivity factors, thereby allowing the rapid quantitation and molecular species fingerprinting of TAG molecular species directly from chloroform extracts of biological samples. (C) 2001 Academic Press.