The chromophore structure of the long-lived intermediate of the C128T channelrhodopsin-2 variant
The chromophore structure of the long-lived intermediate of the C128T channelrhodopsin-2 variant
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DOI：
10.1016/j.febslet.2011.11.007
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发表时间：
2011-12-15
期刊：
影响因子：
3.5
通讯作者：
Hildebrandt, Peter
中科院分区：
文献类型：
--
作者：
Bruun, Sara;Naumann, Hendrik;Hildebrandt, Peter
用共振拉曼光谱研究了光激活通道视紫红质-2 C128T的光循环，重点研究了中间体P380和P353，它们构成了母态恢复的旁路。P353物种显示出UV-Vis吸收光谱，其精细结构使人想起细菌视紫红质的还原形式，而各自的RR光谱有很大不同。相反，P380/P353中间对的RR光谱与全反式构型中的游离视网膜的RR光谱密切相关。这些发现表明，通过P380/P353的亲本状态恢复涉及视网膜-蛋白质连接的瞬时水解和重新形成。(C)2011年欧洲生化学会联合会。爱思唯尔出版公司版权所有。
The photocycle of the light-activated channel, channelrhodopsin-2 C128T, has been studied by resonance Raman (RR) spectroscopy focussing on the intermediates P380 and P353 that constitute a side pathway in the recovery of the parent state. The P353 species displays a UV-vis absorption spectrum with a fine-structure reminiscent of the reduced-retro form of bacteriorhodopsin, whereas the respective RR spectra differ substantially. Instead, the RR spectra of the P380/P353 intermediate couple are closely related to that of a free retinal in the all-trans configuration. These findings imply that the parent state recovery via P380/P353 involves the transient hydrolysis and re-formation of the retinal-protein linkage. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.