Carotenoids of aleurone, germ, and endosperm fractions of barley, corn and wheat differentially inhibit oxidative stress.
Carotenoids of aleurone, germ, and endosperm fractions of barley, corn and wheat differentially inhibit oxidative stress.
复制标题
DOI：
10.1021/jf5058606
复制
发表时间：
2015-03
影响因子：
6.1
通讯作者：
Kabo Masisi;W. Diehl-Jones;J. Gordon;D. Chapman;M. Moghadasian;T. Beta
中科院分区：
文献类型：
--
作者：
Kabo Masisi;W. Diehl-Jones;J. Gordon;D. Chapman;M. Moghadasian;T. Beta
从大麦、玉米和小麦的糊粉层、胚芽和胚乳组分中提取的类胡萝卜素的抗氧化潜力已被评估。HPLC分析证实了在谷物颗粒级分的提取物中存在叶黄素和玉米黄质类胡萝卜素（nd-15139 μg/kg）。采用2，2-二苯基-1-苦基肼、氧自由基吸收能力、2，2 '-连氮基-双（3-乙基苯并噻唑啉-6-磺酸）测定的抗氧化性质表明，胚芽的抗氧化活性显著高于糊粉层和胚乳组分（P<0.001）。用3-（4，5-二甲基噻唑基-2）-2，5-二苯基溴化四氮唑（MTT）比色法，用类胡萝卜素提取物预孵育Caco-2、HT-29和FH 74 Int细胞，可有效地减少2，2 '偶氮二（2-脒基丙烷）二盐酸盐（AAPH）诱导的细胞损失。此外，类胡萝卜素提取物降低（P<0.001）AAPH诱导的细胞内氧化，表明抗氧化活性。芯片分析的84个抗氧化途径基因中，有28个基因表达增强（P<0.05）。我们的研究结果表明，类胡萝卜素的胚芽，糊粉，胚乳馏分提高抗氧化能力，从而有可能减轻氧化应激。
The antioxidant potential of carotenoids from aleurone, germ, and endosperm fractions of barley, corn, and wheat has been evaluated. HPLC analysis confirmed the presence of lutein and zeaxanthin carotenoids (nd-15139 μg/kg) in extracts of cereal grain fractions. The antioxidant properties using 2,2-diphenyl-1-picrylhydrazyl, oxygen radical absorbance capacity, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assays revealed significantly higher (P<0.001) antioxidant activity in the germ than in the aleurone and endosperm fractions. Using 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay, 2,2'azobis (2-amidinopropane)dihydrochloride (AAPH)-induced cell loss was effectively reduced by preincubating Caco-2, HT-29, and FHs 74 Int cells with carotenoid extracts. Moreover, carotenoid extracts reduced (P<0.001) AAPH-induced intracellular oxidation in the cell lines, suggesting antioxidant activity. Of the 84 antioxidant pathway genes included in microarray array analysis (HT-29 cells), the expressions of 28 genes were enhanced (P<0.05). Our findings suggest that carotenoids of germ, aleurone, and endosperm fractions improved antioxidant capacity and thus have the potential to mitigate oxidative stress.