B-SOLANA: an approach for the analysis of two-base encoding bisulfite sequencing data
B-SOLANA: an approach for the analysis of two-base encoding bisulfite sequencing data
复制标题
DOI：
10.1093/bioinformatics/btr660
复制
发表时间：
2012-02-01
期刊：
影响因子：
5.8
通讯作者：
Franke, Andre
中科院分区：
文献类型：
--
作者：
Kreck, Benjamin;Marnellos, George;Franke, Andre
亚硫酸氢盐测序是亚硫酸氢盐处理和高通量测序的组合，已被证明是在单碱基分辨率下测量DNA甲基化的有价值的方法。在这里，我们提出了B-SOLANA，一种在生命技术公司的SOLiD平台上分析双碱基编码（颜色空间）亚硫酸氢盐测序数据的方法。它包括亚硫酸氢盐序列的比对以及CpG和非CpG序列背景中甲基化水平的测定。B-SOLANA能够快速准确地分析大型原始序列数据集。可用性和实现：在GNU GPL v3许可证下发布的源代码可以在http://code.google.com/p/bsolana/上免费获得。
Bisulfite sequencing, a combination of bisulfite treatment and high-throughput sequencing, has proved to be a valuable method for measuring DNA methylation at single base resolution. Here, we present B-SOLANA, an approach for the analysis of twobase encoding (colorspace) bisulfite sequencing data on the SOLiD platform of Life Technologies. It includes the alignment of bisulfite sequences and the determination of methylation levels in CpG as well as non-CpG sequence contexts. B-SOLANA enables a fast and accurate analysis of large raw sequence datasets. Availability and implementation: The source code, released under the GNU GPLv3 licence, is freely available at http://code.google.com/p/bsolana/.