Nanomolar concentrations of Bowman-Birk soybean protease inhibitor suppress x-ray-induced transformation in vitro.
Nanomolar concentrations of Bowman-Birk soybean protease inhibitor suppress x-ray-induced transformation in vitro.
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DOI：
10.1073/pnas.82.16.5395
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发表时间：
1985-08
影响因子：
11.1
通讯作者：
J. Yavelow;M. Collins;Y. Birk;W. Troll;A. Kennedy
中科院分区：
文献类型：
--
作者：
J. Yavelow;M. Collins;Y. Birk;W. Troll;A. Kennedy
这里报道的实验表明，一种粗制大豆提取物，如果用丙酮脱脂，可以有效地阻止体外细胞转化。这种粗提物的活性成分是Bowman-Birk胰蛋白酶和糜蛋白酶抑制剂。Bowman-Birk抑制剂的胰凝乳蛋白酶抑制区域负责抑制体外转化。另一种低分子量大豆胰蛋白酶抑制剂对转化没有明显的抑制作用。鲍曼-伯克抑制剂(I)对转化过程具有不可逆转的影响，(II)即使在致癌物暴露后许多天添加到培养物中也能抑制辐射诱导的转化，以及(III)当其存在于低至0.125 nM的介质中时，其抑制转化的能力是有效的。
Experiments reported here indicate a crude soybean extract, if defatted with acetone, effectively blocks cell transformation in vitro. An active component of this crude extract is the Bowman-Birk trypsin and chymotrypsin inhibitor. The chymotrypsin-inhibitory region of the Bowman-Birk inhibitor is responsible for suppressing in vitro transformation. Another low molecular weight soybean trypsin inhibitor does not significantly suppress transformation. The Bowman-Birk inhibitor (i) has an irreversible effect on the transformation process, (ii) can suppress radiation-induced transformation even when added to cultures many days after the carcinogen exposure, and (iii) is effective in its ability to suppress transformation when present in the medium at a concentration as low as 0.125 nM.