Nonfunctional ingestion of plant miRNAs in silkworm revealed by digital droplet PCR and transcriptome analysis.
Nonfunctional ingestion of plant miRNAs in silkworm revealed by digital droplet PCR and transcriptome analysis.
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DOI：
10.1038/srep12290
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发表时间：
2015-07-21
影响因子：
4.6
通讯作者：
He N
中科院分区：
文献类型：
--
作者：
Jia L;Zhang D;Xiang Z;He N
自从发现植物miRNA（miR 168）交叉调控哺乳动物转录本以来，miRNA介导的跨界通讯已成为最引人注目但又最具争议性的研究课题之一。在本研究中，我们使用家蚕和桑葚，这是一种研究昆虫与其宿主植物之间相互作用的模型，以解决miRNA介导的跨王国通信是否是一种普遍现象。TA克隆、桑格测序和液滴数字PCR结果表明，几种桑源miRNAs可以进入家蚕血淋巴和多种供试组织。在血淋巴和脂肪体中也检测到合成的miR 166 b。然而，通过RNA-seq分析、RT-PCR和表型研究发现，摄食合成的miR 166 b对家蚕的生理进程没有影响。桑葚miRNAs经口转移到家蚕体内，没有发现与miRNAs相关的生理过程。这一结果为miRNA的跨界转移提供了新的视角。
Since a plant miRNA (miR168) cross-regulating a mammalian transcript was reported, miRNA-mediated cross-kingdom communication has become one of the most compelling but controversial topics. In the present study, we used silkworm and mulberry, which is a model for studies on the interactions between the insect and its host plant, to address whether miRNA-mediated cross-kingdom communication is a common phenomenon. The results of TA clone, Sanger sequencing and droplet digital PCR demonstrated that several mulberry-derived miRNAs could enter to silkworm hemolymph and multiple tested tissues. Synthetic miR166b was also detected in hemolymph and fat body. However, the ingestion of synthetic miR166b did not play roles in silkworm physiological progress, which was revealed by RNA-seq analyses, RT-PCR, and phenotypic investigations. Mulberry miRNAs are convincingly transferred to the silkworm orally and no physiological process associated with the miRNAs was demonstrable. The results provided a new aspect of cross-kingdom miRNA transfer.