Bone lengthening osteogenesis, a combination of intramembranous and endochondral ossification: an experimental study in sheep.
Bone lengthening osteogenesis, a combination of intramembranous and endochondral ossification: an experimental study in sheep.
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DOI：
10.1007/s11751-010-0083-y
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发表时间：
2010-08
影响因子：
0.8
通讯作者：
Denaro V
中科院分区：
文献类型：
--
作者：
Forriol F;Denaro L;Longo UG;Taira H;Maffulli N;Denaro V
我们在24只手术时2 - 3个月大的羔羊身上建立了胫骨胼胝体延长实验模型，对新形成组织的形态学特征进行了评估。单侧外固定架原型Monotube Triax®（Stryker Howmedica Osteonics, New Jersey）应用于左胫骨。经皮截骨术以最小创伤方式使用凿子进行。术后7天开始延长至30mm。将24只动物随机分为3组，每组8只：第一组以1 mm/d的速度延长，连续30 d；第二组，以2毫米/天的速率，持续15天；第三组，剂量为3mm /d，连续10天。每组延长结束2周后处死4只，延长结束4周后处死4只。为评估牵张区骨形成情况，自手术当日起每2周拍摄一次x线片。为了研究血管形成的过程，我们使用了Spalteholz技术。宰杀后，取下每只动物的胫骨，切片用苏木精和伊红、马松三色和红花素o染色。免疫组化，使用特异性抗体检测胶原I和II， S100蛋白和纤维连接蛋白。牵张部位同时发生膜内和软骨内成骨。我们的研究提供了绵羊胫骨延长实验模型中新形成组织的详细结构特征，可能对胼胝质的进一步研究有用。
We evaluated the morphological features of the newly formed tissue in an experimental model of tibial callotasis lengthening on 24 lambs, aged from 2 to 3 months at the time of operation. A unilateral external fixator prototype Monotube Triax® (Stryker Howmedica Osteonics, New Jersey) was applied to the left tibia. A percutaneous osteotomy was performed in a minimally traumatic manner using a chisel. Lengthening was started 7 days after surgery and was continued to 30 mm. The 24 animals were randomly divided into three groups of 8 animals each: in Group 1, lengthening took place at a rate of 1 mm/day for 30 days; in Group 2, at a rate of 2 mm/day for 15 days; in Group 3, at a rate of 3 mm/day for 10 days. In each group, 4 animals were killed 2 weeks after end of lengthening, and the other 4 animals at 4 weeks after end of lengthening. To assess bony formation in the distraction area, radiographs were taken every 2 weeks from the day of surgery. To study the process of vascularization, we used Spalteholz’s technique. After killing, the tibia of each animal was harvested, and sections were stained with hematoxylin and eosin, Masson’s trichrome, and Safranin-O. Immunohistochemistry was performed, using specific antibodies to detect collagens I and II, S100 protein, and fibronectin. A combination of intramembranous and endochondral ossification occurred together at the site of distraction. Our study provides a detailed structural characterization of the newly formed tissue in an experimental model of tibial lengthening in sheep and may be useful for further investigations on callotasis.