Preparation and rheological characterization of a gel form of the porcine urinary bladder matrix
Preparation and rheological characterization of a gel form of the porcine urinary bladder matrix
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DOI：
10.1016/j.biomaterials.2007.12.014
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发表时间：
2008-04-01
期刊：
影响因子：
14
通讯作者：
Badylak, Stephen F.
中科院分区：
文献类型：
--
作者：
Freytes, Donald O.;Martin, Jeffrey;Badylak, Stephen F.
在临床和临床前研究中，由细胞外基质（ECM）组成的生物支架已被用于促进各种组织的修复和重建。这种支架的临床应用可能受到从中收获ECM的组织或器官的几何和机械性质的限制。可注射凝胶形式的细胞外基质可能符合任何三维形状，并可以通过微创技术输送到感兴趣的部位。本研究的目的是制备从膀胱（膀胱基质或UBM）收获的ECM的凝胶形式，表征凝胶的流变学性质，并最终评估凝胶支持平滑肌细胞体外生长的能力。在用胃蛋白酶酶促溶解后，当带到生理条件下时，诱导UBM自组装成凝胶。UBM凝胶支持大鼠主动脉平滑肌细胞在静态体外培养条件下的粘附和生长。目前的研究表明，完整形式的UBM可以成功地溶解，而无需纯化步骤，并诱导其重新溶解成凝胶形式的UBM生物支架材料。(c)2007爱思唯尔有限公司版权所有。
Biologic scaffolds composed of extracellular matrix (ECM) have been used to facilitate the repair and reconstruction of a variety of tissues in clinical and pre-clinical studies. The clinical utility of such scaffolds can be limited by the geometric and mechanical properties of the tissue or organ from which the ECM is harvested. An injectable gel form of ECM could potentially conform to any three-dimensional shape and could be delivered to sites of interest by minimally invasive techniques. The objectives of the present study were to prepare a gel form of ECM harvested from the urinary bladder (urinary bladder matrix or UBM), to characterize the rheological properties of the gel, and finally to evaluate the ability of the gel to support in vitro growth of smooth muscle cells. Following enzymatic solubilization with pepsin, UBM was induced to self-assemble into a gel when brought to physiological conditions. The UBM gel supported the adhesion and growth of rat aortic smooth muscle cells when cultured under static in vitro conditions. The present study showed that an intact form of UBM can be successfully solubilized without purification steps and induced to repolymerize into a gel form of the UBM biologic scaffold material. (c) 2007 Elsevier Ltd. All rights reserved.