Genome-wide identification of laccase gene family in three Phytophthora species
Genome-wide identification of laccase gene family in three Phytophthora species
复制标题
DOI：
10.1007/s10709-012-9696-z
复制
发表时间：
2012-12-01
期刊：
影响因子：
1.5
通讯作者：
Li, Peiqian
中科院分区：
文献类型：
--
作者：
Feng, Baozhen;Li, Peiqian
疫霉属是卵菌纲中的主要病原体，引起植物的经济和环境破坏性流行病。漆酶广泛存在于生活的各个领域，但在卵菌中未见报道。本文对疫霉菌漆酶基因进行了研究。在辣椒疫霉、大豆疫霉和多枝疫霉3个基因组中鉴定出了3个致病菌。共鉴定出18个漆酶基因，其中辣椒疫霉基因组4个，大豆疫霉基因组6个，多枝疫霉基因组8个。大多数预测的基因模型具有典型的真菌漆酶特征，具有3个保守位点，其中1个半胱氨酸和10个组氨酸残基。系统发育分析表明，疫霉属漆酶聚为4个分支，而真菌漆酶聚在一起。本研究结果为卵菌漆酶作用的新假说提供了理论依据，并对今后的研究具有指导意义。
Phytophthora spp. is a primary pathogen in oomycete, causing economically and environmentally devastating epidemics of plants. Laccases have been found in all domains of life but have not been reported in oomycte. In this paper, laccase genes of Phytophthora spp. were identified in three genomes (Phytophthora capsici, Phytophthora sojae and Phytophthora ramorum). 18 laccase genes were identified in total, including four in P. capsici genome, six in P. sojae genome and eight in P. ramorum genome. Most of the predicted gene models shared typical fungal laccase character, possessing three conserved positions with one cysteine and ten histidine residues at these positions. Phylogenetic analysis illustrated that laccases from Phytophthora clustered into four clades, while fungal laccases clustered together. The results provided the theoretical ground for new hypotheses about the roles laccases in oomycetes and may guide the future research of these enzymes.