MiOXSYS: a novel method of measuring oxidation reduction potential in semen and seminal plasma
MiOXSYS: a novel method of measuring oxidation reduction potential in semen and seminal plasma
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DOI：
10.1016/j.fertnstert.2016.05.013
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发表时间：
2016-09-01
影响因子：
6.7
通讯作者：
Sabanegh, Edmund
中科院分区：
文献类型：
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作者：
Agarwal, Ashok;Sharma, Rakesh;Sabanegh, Edmund
目的：测量精液和精浆中的氧化还原电位（ORP）并建立其参考水平。设计：测量精液和精浆中的ORP水平。设置：三级医院。受试者：26名对照和33名不育男性。干预：无。主要结果测量：在时间0和120分钟测量精液和精浆中的静态ORP（sORP）和电容ORP（cORP）。在[1]精液和精浆与[2]时间0和120分钟之间评估ORP的相关性。在（a）对照组和（B）不育患者中研究与精子参数的关联，并产生受试者操作特征曲线以建立sORP cutoff.结果：精液sORP和cORP水平与时间0和时间120分钟的精浆水平相关。在对照组和不育患者中，在时间0和120分钟时，sORP水平与精液以及精浆中的精子浓度和总精子数呈反比关系。基于精子活力的受试者分类显示，具有异常活力的受试者在时间120分钟时表现出较差的浓度、总计数、形态以及精液和精浆sORP水平升高。基于活力的精液和精浆sORP临界值分别为1.48和2.09，能够区分精液质量正常和精液质量异常的受试者。ORP水平不受精液年龄的影响，使这项新技术易于在临床环境中使用。(C)2016年，美国生殖医学会（American Society for Reproductive Medicine）
Objective: To measure oxidative reduction potential (ORP) in semen and seminal plasma and to establish their reference levels.Design: ORP levels were measured in semen and seminal plasma.Setting: Tertiary hospital.Subject(s): Twenty-six controls and 33 infertile men.Intervention(s): None.Main Outcome Measure(s): Static ORP (sORP) and capacitance ORP (cORP) were measured in semen and seminal plasma at time 0 and 120 minutes. Correlation of ORP was assessed between [1] semen and seminal plasma and [2] time 0 and 120 minutes. The association with sperm parameters was studied in (a) controls and (b) infertile patients, and a receiver operating characteristic curve was generated to establish the sORP cutoff.Result(s): Semen sORP and cORP levels were associated with seminal plasma levels at time 0 and time 120 minutes. In controls and infertile patients, an inverse relationship of sORP levels was established with concentration and total sperm count in semen as well as seminal plasma at time 0 and 120 minutes. Classification of subjects based on sperm motility showed that subjects with abnormal motility present with poor concentration, total count, morphology, and elevated levels of semen and seminal plasma sORP at time 120 minutes. The sORP cutoff of 1.48 in semen and 2.09 in seminal plasma based on motility was able to distinguish subjects with normal semen quality from those with abnormal semen quality.Conclusion(s): The MiOXSYS System can reliably measure ORP levels in semen and seminal plasma. ORP levels are not affected by semen age, making this new technology easy to employ in a clinical setting. (C) 2016 by American Society for Reproductive Medicine.