Transcriptional regulation of three EIN3-like genes of carnation (Dianthus caryophyllus L. cv. Improved White Sim) during flower development and upon wounding, pollination, and ethylene exposure
Transcriptional regulation of three EIN3-like genes of carnation (Dianthus caryophyllus L. cv. Improved White Sim) during flower development and upon wounding, pollination, and ethylene exposure
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DOI：
10.1093/jxb/eri199
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发表时间：
2005-08-01
影响因子：
6.9
通讯作者：
Verlinden, S
中科院分区：
文献类型：
--
作者：
Iordachescu, M;Verlinden, S
采用多种方法从香石竹（Dianthuscaryophyllus）花瓣中分离到3个EIL基因DC-EIL 1/2（AY 728191）、DC-EIL 3（AY 728192）和DC-EIL 4（AY 728193）。DC-EIL 1/2推导的氨基酸序列与先前克隆和表征的香石竹DC-EIL 1（AF 261654）具有98%的同一性，与DC-EIL 3具有62%的同一性，与DC-EIL 4具有60%的同一性。DC-EIL 3推导的氨基酸序列与先前克隆的香石竹基因片段Dc 106（CF259543）具有100%的同源性，与香石竹DC-EIL 1（AF 261654）具有61%的同源性，与DC-EIL 4具有59%的同源性。DC-EIL 4与DC-EIL 1（AF 261654）具有60%的同一性。营养和花组织（花瓣，子房，和风格）在生长发育和衰老（自然和乙烯诱导）的表达分析表明，在康乃馨的DC-EIL家族的基因的mRNA积累调节发育和乙烯。DC-EIL 3 mRNA在乙烯暴露后、花发育过程中以及授粉后在花瓣和花柱中均有显著积累。有趣的是，当乙烯水平增加时，在受伤的叶片和衰老花的子房中发现DC-EIL 3 mRNA的水平降低。与对照花相比，用蔗糖处理的花表现出2天延迟DC-EIL 3转录物的积累。这些观察结果表明DC-EIL 3在生长和发育过程中的重要作用。DC-EIL 1/2和DC-EIL 4 mRNA水平的变化在花发育过程中，乙烯暴露和授粉后非常相似。授粉后花柱中DC-EILs的mRNA水平与乙烯释放量呈正相关。在本研究中，EIN 3样基因的克隆和表征表明它们的转录调控以前没有观察到EIL。
Using a combination of approaches, three EIN3-like (EIL) genes DC-EIL1/2 (AY728191), DC-EIL3 (AY728192), and DC-EIL4 (AY728193) were isolated from carnation (Dianthus caryophyllus) petals. DC-EIL1/2 deduced amino acid sequence shares 98% identity with the previously cloned and characterized carnation DC-EIL1 (AF261654), 62% identity with DC-EIL3, and 60% identity with DC-EIL4. DC-EIL3 deduced amino acid sequence shares 100% identity with a previously cloned carnation gene fragment, Dc106 (CF259543), 61% identity with Dianthus caryophyllus DC-EIL1 (AF261654), and 59% identity with DC-EIL4. DC-EIL4 shared 60% identity with DC-EIL1 (AF261654). Expression analyses performed on vegetative and flower tissues (petals, ovaries, and styles) during growth and development and senescence (natural and ethylene-induced) indicated that the mRNA accumulation of the DC-EIL family of genes in carnation is regulated developmentally and by ethylene. DC-EIL3 mRNA showed significant accumulation upon ethylene exposure, during flower development, and upon pollination in petals and styles. Interestingly, decreasing levels of DC-EIL3 mRNA were found in wounded leaves and ovaries of senescing flowers whenever ethylene levels increased. Flowers treated with sucrose showed a 2 d delay in the accumulation of DC-EIL3 transcripts when compared with control flowers. These observations suggest an important role for DC-EIL3 during growth and development. Changes in DC-EIL1/2 and DC-EIL4 mRNA levels during flower development, and upon ethylene exposure and pollination were very similar. mRNA levels of the DC-EILs in styles of pollinated flowers showed a positive correlation with ethylene production after pollination. The cloning and characterization of the EIN3-like genes in the present study showed their transcriptional regulation not previously observed for EILs.