Temperature dependence of endocytosis in renal epithelial cells in culture
Temperature dependence of endocytosis in renal epithelial cells in culture
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DOI：
10.1016/0005-2736(96)00077-6
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发表时间：
1996-07-25
影响因子：
3.4
通讯作者：
LeGrimellec, C
中科院分区：
文献类型：
--
作者：
Mamdouh, Z;Giocondi, MC;LeGrimellec, C
在两个肾上皮细胞系，MDCK细胞和LLC-PK 1细胞，使用荧光黄或辣根过氧化物酶作为标记物的液相内吞的温度依赖性进行了测定。对于在固体支持物上生长为汇合单层的两种细胞系，获得了标记物摄取与温度的双相曲线。斜率的变化发生在27 ℃左右，这是MDCK细胞质膜脂质进入凝胶状态的临界温度。高于27 ℃时的活化能（15-22 kcal/mol）明显高于低于临界温度时的活化能（9-12 kcal/mol）。这些数据表明，膜物理状态的变化对内吞过程有显着的影响。他们认为，具有不同活化能的两种机制参与了培养的肾上皮细胞的液相内吞作用。
Temperature dependence of fluid-phase endocytosis was determined in two renal epithelial cell lines, MDCK cells and LLC-PK1 cells, using Lucifer Yellow or horseradish peroxidase as markers. For both cell lines, grown on solid support as a confluent monolayer, biphasic curves of marker uptake vs. temperature were obtained. The changes in slope occurred around 27 degrees C, a critical temperature at which the lipids of the plasma membrane of MDCK cells enter in the gel state. Activation energies were significantly higher above 27 degrees C (15-22 kcal/mol) than below that critical temperature (9-12 kcal/mol). These data indicate that changes in membrane physical state have marked effects on endocytic processes. They suggest that two mechanisms, with different activation energies are involved in the fluid phase endocytosis by renal epithelial cells in culture.