Purification and characterization of the androgen receptor from rat ventral prostate.
Purification and characterization of the androgen receptor from rat ventral prostate.
复制标题
大鼠腹侧前列腺雄激素受体的纯化和表征。
DOI：
10.1021/bi00295a020
复制
发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
Tindall,DJ
中科院分区：
文献类型：
--
作者：
Chang,CH;Rowley,DR;Tindall,DJ
Ching H. Chang, David R. Rowley和Donald J. Tindall*摘要：采用差异DNA-Sepharose 4B层析和睾酮17/3-半琥珀基- 3,3 '-二氨基二丙胺- sepharose 4B有限层析相结合的方法从大鼠前列腺腹侧细胞质中纯化雄激素受体。从38 g大鼠腹侧前列腺中获得约8 ug蛋白质，产率为24%。受体被纯化约120000倍。十二烷基硫酸钠(NaDodS04)-聚丙烯酰胺凝胶的硝酸银染色显示一个主要的多肽带在86000道尔顿迁移。部分纯化受体制备的17-羟基-17 a-[3H]甲基- 4,9,1 -雌二醇-3- 1或17/3-羟基-亲和标记
Ching H. Chang, David R. Rowley, and Donald J. Tindall* abstract: The androgen receptor has been purified from rat ventral prostate cytosol by a combination of differential DNA-Sepharose 4B chromatography and testosterone 17/3-hemisuccinyl-3, 3'-diaminodipropylamine-Sepharose 4B af-finity chromatography. Approximately 8 ug of protein was obtained from 38 g of rat ventral prostate, with a yield of 24%. The receptor was purified approximately 120000-fold. Silver nitrate staining of a sodium dodecyl sulfate (NaDodS04)-polyacrylamide gel revealed a major polypeptide band migrating at 86000 daltons. Affinity labeling of a partially purified receptor preparation with either 17-hydroxy-17 a-[3H] methyl-4, 9, l l-estratrien-3-one or 17/3-hydroxy-