Escherichia coli O157:H7 detection limit of millimeter-sized PZT cantilever sensors is 700 Cells/mL
Escherichia coli O157:H7 detection limit of millimeter-sized PZT cantilever sensors is 700 Cells/mL
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DOI：
10.2116/analsci.21.355
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发表时间：
2005-04-01
影响因子：
1.6
通讯作者：
Mutharasan, R
中科院分区：
文献类型：
--
作者：
Campbell, GA;Mutharasan, R
制备了一种复合自激毫米尺寸的锆钛酸铅（PZT）玻璃悬臂梁（2 mm × 1.8 mm，传感面积6 mm(2)），用于检测大肠杆菌（E. coli） O157:H7。在空气中的基模共振和第二模共振分别为10.95 +/- 0.05 kHz和43.45 +/- 0.05 kHz。亲和纯化单克隆抗体(anti-E。将病原菌大肠杆菌O157:H7特异性的大肠杆菌O157:H7固定在悬臂玻璃尖端，然后浸入含有病原菌的液体（70 ~ 7 × 107个细胞/mL）中。谐振频率降低，达到0 +/- 5、46 +/- 5、260 +/- 5和10105hz的稳态位移，对应于0、700、7000和7 × 10(7) cells/mL。通过实验，该传感器的检出限为700 cells/mL。
A composite self-excited millimeter-sized lead zirconate titanate (PZT) glass cantilever (2 mm x 1.8 mm; sensing area of 6 mm(2)) was fabricated for the detection of Escherichia coli (E. coli) O157:H7. The fundamental and second mode resonance in air was 10.95 +/- 0.05 kHz and 43.45 +/- 0.05 kHz, respectively. Affinity purified monoclonal antibody (anti-E. coli O157:H7) specific to the pathogen E. coli O157:H7 was immobilized at the cantilever glass tip, and then immersed in liquid containing the pathogen (70 to 7 x 107 cells/mL). The resonant frequency showed a reduction and reached a steady state shift of 0 +/- 5, 46 +/- 5, 260 +/- 5, and 1010 5 Hz corresponding to 0, 700, 7000, and 7 x 10(7) cells/mL. From the experiments conducted, the detection limit of the sensor was 700 cells/mL.