Phorbol ester administration transiently increases aromatic L-amino acid decarboxylase activity of the mouse striatum and midbrain.
Phorbol ester administration transiently increases aromatic L-amino acid decarboxylase activity of the mouse striatum and midbrain.
复制标题
佛波酯给药可暂时增加小鼠纹状体和中脑的芳香族 L-氨基酸脱羧酶活性。
DOI：
10.1046/j.1471-4159.1994.63020694.x
复制
发表时间：
1994
影响因子：
4.7
通讯作者：
Hadjiconstantinou,M
中科院分区：
文献类型：
--
作者：
Young,EA;Neff,NH;Hadjiconstantinou,M
Aromaticl‐amino acid decarboxylase (AAAD) is required for the synthesis of catecholamines, serotonin, and the trace amines. We found that the protein kinase C activator phorbol 12‐myristate 13‐acetate administered intracerebroventricularly transiently increased AAAD activity by 30–50% over control values within ∼30 min in the striatum and midbrain of the mouse. The enzyme increase was manifested as an apparent increase ofVmaxwith little change ofKmfor eitherl‐3,4‐dihydroxyphenylalanine or pyridoxal phosphate. Chelerythrine, a protein kinase C inhibitor, prevented the phorbol ester‐induced increase of AAAD. Moreover, okadaic acid, a serine/threonine‐selective protein phosphatase 1 and 2A inhibitor, also increased AAAD activity in the mouse striatum and midbrain. Taken together, these observations suggest that protein kinase C‐mediated pathways modulate AAAD activity in vivo.
