Bio-interaction of nano and bulk lanthanum and ytterbium oxides in soil system: Biochemical, genetic, and histopathological effects on Eisenia fetida
Bio-interaction of nano and bulk lanthanum and ytterbium oxides in soil system: Biochemical, genetic, and histopathological effects on Eisenia fetida
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DOI：
10.1016/j.jhazmat.2021.125574
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发表时间：
2021-03-21
影响因子：
13.6
通讯作者：
Rui, Yukui
中科院分区：
文献类型：
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作者：
Adeel, Muhammad;Shakoor, Noman;Rui, Yukui
稀土元素（REE）在电子工业中的大量应用导致其不可避免地释放到环境中；然而，其对土壤生物群的影响在很大程度上仍未得到解决。我们研究了纳米和散装 La2O3 和 Yb2O3 的恶臭艾美耳球解毒潜力及其在 50、100、200、500 和 1000 mg kg-1 浓度下对生化和遗传标记的潜在影响。我们发现，蚯蚓在中低水平时可以生物修复 3-15% La2O3 和 Yb2O3 污染的土壤，而这种潜力在较高水平时受到限制。纳米和块状 La2O3 和 Yb2O3 处理在 500 和 1000 mg kg-1 下分别抑制乙酰胆碱酯酶 49-65% 和 22-36%，从而诱导蚯蚓神经毒性。纳米 La2O3 被证明是非常有害的，主要是通过氧化应激和随后的抗氧化系统失效。 100 mg·kg-1的纳米La2O3和Yb2O3使亲代和子代蚯蚓中annetocin mRNA的表达显着下调50%和20%，这对蚯蚓的繁殖至关重要。同样，在中等暴露水平下，热休克蛋白 70 (HSP70) 和金属硫蛋白的表达水平在两代中均显着上调。组织学观察表明，200 mg kg-1 的纳米 REE 会引起恶臭艾美耳球菌的肠上皮和伤寒菌发生剧烈变化。迄今为止，我们的研究结果增强了人们对稀土元素和蚯蚓之间相互作用的理解。
The massive application of rare earth elements (REEs) in electronic industries cause their inevitable release into the environment; however, its effects on soil biota remain largely unaddressed. We investigated the E. fetida detoxification potential of nano and bulk La2O3 and Yb2O3 and their potential impact on biochemical and genetic markers at 50, 100, 200, 500 and 1000 mg kg- 1 concentration. We found that earthworms bioremediate 3-15% La2O3 and Yb2O3 contaminated soil at low and medium levels, while this potential was limited at higher levels. Nano and bulk La2O3 and Yb2O3 treatment induced neurotoxicity in earthworm by inhibiting acetylcholinesterase by 49-65% and 22-36% at 500 and 1000 mg kg- 1, respectively. Nano La2O3 proved to be highly detrimental, mainly through oxidative stress and subsequent failure of antioxidant system. Nano La2O3 and Yb2O3 at 100 mg kg- 1 significantly down-regulated the expression of annetocin mRNA in the parental and progeny earthworms by 50% and 20%, which is crucial for earthworm reproduction. Similarly, expression level of heat shock protein 70 (HSP70) and metallothionein was significantly upregulated in both generations at medium exposure level. Histological observations showed that nano REEs at 200 mg kg- 1 induced drastic changes in the intestinal epithelium and typhlosole of E. fetida. To date, our results enhance the understanding of interaction between REEs and earthworms.