Detection of mecC-Positive Staphylococcus aureus (CC130-MRSA-XI) in Diseased European Hedgehogs (Erinaceus europaeus) in Sweden
Detection of mecC-Positive Staphylococcus aureus (CC130-MRSA-XI) in Diseased European Hedgehogs (Erinaceus europaeus) in Sweden
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DOI：
10.1371/journal.pone.0066166
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发表时间：
2013-06-12
期刊：
影响因子：
3.7
通讯作者：
Ehricht, Ralf
中科院分区：
文献类型：
--
作者：
Monecke, Stefan;Gavier-Widen, Dolores;Ehricht, Ralf
最近，在金黄色葡萄球菌中发现了赋予β-内酰胺抗性的新mec基因。该基因，mecC，位于SCCmec XI元件上，该元件迄今已在克隆复合物49、130、425、599和1943中鉴定。目前已知的一些分离株已从动物中鉴定出来。这一点以及对不赋予β-内酰胺耐药性的mecA等位基因的观察表明，mec基因可能在来自动物的葡萄球菌属中具有储库。因此，筛选mec基因的野生分离物也很重要。在这里，我们描述了mecC阳性金黄色葡萄球菌（ST 130-MRSA-XI）和病变相关的感染在两个患病的自由放养的欧洲刺猬（刺猬欧洲）。2003年在瑞典中部发现了一只死于S。金黄色葡萄球菌败血症另一只于2011年在波罗的海的哥特兰岛发现，表现出严重的皮炎，并被安乐死。ST 130-MRSA-XI分离株从来自两种刺猬的病变中分离，并且与先前描述的来自人类的分离株基本相同。两种分离物均携带完整的SCCmec XI元件。它们缺乏lukF-PV/lukS-PV和lukM/lukF-P83基因，但具有先前从猪葡萄球菌、假中间葡萄球菌和其他S. CC 130谱系的金黄色葡萄球菌。据我们所知，这是第一次报告的病例CC 130-MRSA-XI刺猬。鉴于其中一个样本早在2003年就被采集，这是瑞典最早发现该菌株和mecC的。这一结果和其他几个最近的观察结果表明，CC 130可能是一个人畜共患的S。金黄色葡萄球菌和SCCmec XI/mecC可能起源于动物病原体。
Recently, a novel mec gene conferring beta-lactam resistance in Staphylococcus aureus has been discovered. This gene, mecC, is situated on a SCCmec XI element that has to date been identified in clonal complexes 49, 130, 425, 599 and 1943. Some of the currently known isolates have been identified from animals. This, and observations of mecA alleles that do not confer beta-lactam resistance, indicate that mec genes might have a reservoir in Staphylococcus species from animals. Thus it is important also to screen wildlife isolates for mec genes. Here, we describe mecC-positive Staphylococcus aureus (ST130-MRSA-XI) and the lesions related to the infection in two diseased free-ranging European hedgehogs (Erinaceus europaeus). One was found dead in 2003 in central Sweden, and suffered from S. aureus septicaemia. The other one, found on the island of Gotland in the Baltic Sea in 2011, showed a severe dermatitis and was euthanised. ST130-MRSA-XI isolates were isolated from lesions from both hedgehogs and were essentially identical to previously described isolates from humans. Both isolates carried the complete SCCmec XI element. They lacked the lukF-PV/lukS-PV and lukM/lukF-P83 genes, but harboured a gene for an exfoliative toxin homologue previously described from Staphylococcus hyicus, Staphylococcus pseudintermedius and other S. aureus of the CC130 lineage. To the best of our knowledge, these are the first reported cases of CC130-MRSA-XI in hedgehogs. Given that one of the samples was taken as early as 2003, this was the earliest detection of this strain and of mecC in Sweden. This and several other recent observations suggest that CC130 might be a zoonotic lineage of S. aureus and that SCCmec XI/mecC may have originated from animal pathogens.