ISOLATION, STRUCTURE, AND ABSOLUTE CONFIGURATION OF A NEW NATURAL INSECT JUVENILE-HORMONE FROM MANDUCA-SEXTA - (ORGAN CULTURE CORPORA-ALLATA HIGH-RESOLUTION LIQUID CHROMATOGRAPHY TOBACCO HORNWORM)
ISOLATION, STRUCTURE, AND ABSOLUTE CONFIGURATION OF A NEW NATURAL INSECT JUVENILE-HORMONE FROM MANDUCA-SEXTA - (ORGAN CULTURE CORPORA-ALLATA HIGH-RESOLUTION LIQUID CHROMATOGRAPHY TOBACCO HORNWORM)
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DOI：
10.1073/pnas.70.5.1509
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发表时间：
1973-01-01
影响因子：
11.1
通讯作者：
SIDDALL, JB
中科院分区：
文献类型：
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作者：
JUDY, KJ;SCHOOLEY, DA;SIDDALL, JB
从烟草天蛾的咽侧体器官培养物中分离出两种保幼激素，并通过高分辨率液相色谱法纯化。它们分别被鉴定为（2 E，6 E）-（10 R）-10，11-环氧-3，7，11-三甲基-2，6-十二碳二烯酸甲酯和（2 E，6 E）-（10 R，11 S）-10，11-环氧-3，7，11-三甲基-2，6-十三碳二烯酸甲酯。[14 C]甲硫氨酸仅结合到其甲氧羰基中。详细介绍了其体外技术和结构的化学证明。本文讨论了一种新的保幼激素出现的意义以及生产、分离和鉴定的新技术。
Two juvenile hormones are isolated from organ cultures of corpora allata of the tobacco hornworm moth,Manduca sextaJohannson, and are purified by high-resolution liquid chromatography. These are identified as methyl (2E, 6E)-(10R)-10,11-epoxy-3,7,11-trimethyl-2,6-dodecadienoate, a new natural hormone, and methyl (2E,6E) - (10R,11S) - 10,11 - epoxy - 3,7,11 - trimethyl-2,6-tridecadienoate. [14C]Methionine is incorporated only into their methoxycarbonyl group. Details of thein vitrotechniques and the chemical proof of structures are presented. The significance of the occurrence of a new juvenile hormone and the new techniques used for production, isolation, and identification are discussed.