Seasonality effect on the composition of oxindole alkaloids from distinct organs of Uncaria tomentosa from the Caribbean region of Costa Rica
Seasonality effect on the composition of oxindole alkaloids from distinct organs of Uncaria tomentosa from the Caribbean region of Costa Rica
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DOI：
10.1016/j.phytochem.2018.03.008
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发表时间：
2018-07-01
期刊：
影响因子：
3.8
通讯作者：
Aguilar-Cascante, Francisco
中科院分区：
文献类型：
--
作者：
Alvarenga-Venutolo, Silvana;Rosales-Lopez, Catalina;Aguilar-Cascante, Francisco
野钩藤（野生）dc (Rubiaceae)，俗称“Una de Gato”或“猫爪”，是一种来自热带雨林的热带藤本植物，用于传统医学，遍布中美洲和南美洲，包括哥斯达黎加。人们对主要由氧吲哚生物碱（OA）具有生物活性的药用提取物的需求日益增加，其中四环（TOA）和五环氧吲哚生物碱（POA）的比值决定了其药用可行性。该比率受不同因素的影响，包括季节环境条件的动态。本研究的目的是评估哥斯达黎加加勒比地区生长的毛毛藤植物器官中氧吲哚生物碱含量的季节性影响。各季节总OA值以幼叶次之，成熟叶次之；其中，异紫茶碱、翼龙柏碱和异曲霉碱是主要的OA。两种叶片材料的POA/TOA比值接近1:1 (3.2 mg g（-1): 3.1 mg g(-1)）。树皮和根在四季均呈五环化学型，比例为6:1 (6.7 mg g(-1): 1.3 mg g(-1))，主要POA为翼龙柏碱和异曲霉碱。幼叶、树皮和根的POA含量具有明显的季节性，从雨季到旱季显著增加。相比之下，TOA含量在所有植物部位几乎保持不变。除降水外，各季节的湿度和温度基本不变，反映了水分含量的差异对POA量的影响。进一步研究非生物因素，如水分胁迫，可以解释POA含量的季节性变化。(C) 2018 Elsevier Ltd.版权所有。
Uncaria tomentosa (Willd.) D.C. (Rubiaceae), commonly known as "Una de Gato" or "Cat's Claw", is a tropical vine from the rainforest used in traditional medicine and spread through Central and South America, including Costa Rica. There is an increasing demand for medicinal extracts with biological activity attributed mainly to oxindole alkaloids (OA), where the ratio between tetracyclic (TOA) and pentacyclic oxindole alkaloids (POA) determines its feasibility for medicinal applications. The ratio is affected by distinct factors including the dynamics of environmental conditions during seasons. The purpose of the study was to assess the seasonality effect in oxindole alkaloids content in relation to plant organs from U. tomentosa grown in the Caribbean region of Costa Rica. Young leaves followed by mature leaves presented the highest amount of total OA during seasons; for these, isoryncophylline, pteropodine and isomitraphylline, were the predominant OA. The POA/TOA ratio of both leaf materials was nearly 1:1 (3.2 mg g(-1): 3.1 mg g(-1)). Bark and root material showed a pentacyclic chemotype in all seasons with a ratio of 6:1 (6.7 mg g(-1) : 1.3 mg g(-1)) with pteropodine and isomitraphylline as the predominant POA. The POA content presented seasonality with a significant increase from rainy to dry season in young leaves, bark and roots. In contrast, TOA amount remained virtually unchanged in all plant parts. Humidity and temperature between the studied seasons were constant except for precipitation, reflecting that differences of water content had an effect in the POA amounts. Further studies of abiotic factors, like water stress, could explain the variation of POA content due to seasonality. (C) 2018 Elsevier Ltd. All rights reserved.