A specific genetic alteration on chromosome 6 in ulcerative colitis-associated colorectal cancers.
A specific genetic alteration on chromosome 6 in ulcerative colitis-associated colorectal cancers.
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DOI：
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发表时间：
2003-07
期刊：
影响因子：
11.2
通讯作者：
T. Ezaki;Mamoru Watanabe;N. Inoue;T. Kanai;H. Ogata;Y. Iwao;H. Ishii;T. Hibi
中科院分区：
文献类型：
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作者：
T. Ezaki;Mamoru Watanabe;N. Inoue;T. Kanai;H. Ogata;Y. Iwao;H. Ishii;T. Hibi
溃疡性结肠炎（UC）相关的结直肠癌（CRC）中的各种遗传变异已被报道。然而，几乎所有的研究都显示了已知基因的异常，这些基因在散发性CRC中得到了证实。为了发现新的遗传变异，我们无意中选择了35个微卫星标记，并在CRC或UC的异型增生病变中进行等位基因型研究。6号染色体（D 6S 468）上杂合性丢失（洛; 62.5%）的频率较高，而其它染色体上均未检测到杂合性丢失。在D 6S 468位点周围有4个额外的微卫星标记，我们确定了UC相关CRC和异型增生中D 6S 1543和D 6S 1580两个位点之间的常见缺失区域。有趣的是，在散发性CRC和长期广泛UC的严重炎症病变中，在该区域没有洛缺失。这些结果表明，在6号染色体上存在新的肿瘤抑制基因与UC的癌变有关，但与散发性CRC无关。
Various genetic alterations in ulcerative colitis (UC)-associated colorectal cancers (CRCs) have been reported. However, almost all studies have shown abnormalities of the known genes that have been demonstrated in sporadic CRCs. To identify novel genetic alterations, we selected unintentionally 35 microsatellite markers, and performed allelotype study in CRCs or dysplastic lesions from UC. High frequency of loss of heterozygosity (LOH; 62.5%) was detected on chromosome 6 (D6S468) but not on other chromosomes. With four additional microsatellite markers around the D6S468 locus, we determined the commonly deleted region between two loci, D6S1543 and D6S1580, in UC-associated CRCs and dysplasia. Interestingly, there was no LOH in this region in sporadic CRCs and severely inflamed lesions of longstanding and extensive UC without cancers. These results indicated the presence of novel tumor suppressor genes on chromosome 6 related to the carcinogenesis of UC but not to sporadic CRCs.