Acoustic droplet vaporization for temporal and spatial control of tissue occlusion: A kidney study
Acoustic droplet vaporization for temporal and spatial control of tissue occlusion: A kidney study
复制标题
DOI：
10.1109/tuffc.2005.1503996
复制
发表时间：
2005-07-01
影响因子：
3.6
通讯作者：
Fowlkes, JB
中科院分区：
文献类型：
--
作者：
Kripfgans, OD;Orifici, CM;Fowlkes, JB
声学液滴汽化（ADV）已被引入通过闭塞和随后的坏死的肿瘤治疗的潜在应用。麻醉新西兰白色兔，将其左肾取出。将成像阵列和单元件换能器定位在水箱中，直接进入肾脏的脉管系统和肾动脉。在肾动脉声穿透期间，将过滤的液滴乳剂（直径[6 μ m]）动脉内（IA）注射到左心脏中，并将ADV引起的血流减少程度与未处理的右肾进行比较。肾组织样品和来自股动脉的参考血液的流式细胞术（使用有色微球）允许定量估计局部血流量。使用ADV，治疗区域的最大局部血流量减少> 90%，平均器官灌注减少> 70%。左肾治疗后，对侧对照肾显示局部血流量相对于ADV前基线最大减少18%。监测来自ADV或IA注射的基于图像的高回声性约90分钟，并且皮质灌注减少其原始值的> 60%超过1小时。这可能是足够的时间开始细胞死亡和可能的肿瘤治疗通过缺血性坏死。此外，目前使用的基于射频组织消融的肿瘤治疗可以受益于ADV，因为通过血管冷却减少了热损失。
Acoustic droplet vaporization (ADV) has been introduced with the potential application of tumor treatment via occlusion and subsequent necrosis. New Zealand White rabbits were anesthetized, and their left kidney was externalized. An imaging array and single-element transducer were positioned in a tank with direct access to the kidney's vasculature and renal artery. Filtered droplet emulsions (diameter [6 mu m) were injected intra-arterially (IA) into the left heart during insonification of the renal artery, and the extent of blood flow reduction by ADV was compared to the untreated right kidney. Flow cytometry (using colored microspheres) of kidney tissue samples and reference blood from the femoral artery allowed the quantitative estimation of regional blood flow. A maximum regional blood flow reduction in the treated region of > 90% and an average organ perfusion reduction of > 70% was achieved using ADV. After treatment of the left kidney, the control kidney on the contralateral side showed a maximum decrease in regional blood flow of 18% relative to the pre-ADV baseline. Image-based hyper-echogenicity from ADV of IA injections was monitored for approximately 90 minutes, and cortex perfusion was reduced by > 60% of its original value for more than 1 hour. This could be enough time for the onset of cell death and possible tumor treatment via ischemic necrosis. Moreover, currently used radio-frequency tissue ablation-based tumor treatment could benefit from ADV due to the decreased heat loss via vascular cooling.