Time resolved fluorescence tomography of turbid media based on lifetime contrast
Time resolved fluorescence tomography of turbid media based on lifetime contrast
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DOI：
10.1364/oe.14.012255
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发表时间：
2006-12-11
期刊：
影响因子：
3.8
通讯作者：
Bacskai, Brian J.
中科院分区：
文献类型：
--
作者：
Kumar, Anand T. N.;Raymond, Scott B.;Bacskai, Brian J.
时域（TD）荧光层析成像的一般线性模型，允许从混浊介质的整个时间荧光响应的寿命为基础的分析。模拟结果表明，TD荧光层析成像是最佳执行使用两个互补的方法：直接TD分析的几个时间点附近的峰值的时间响应，这提供了上级分辨率;以及基于时间响应的衰减部分的渐近多指数分析的层析成像，其为成像介质中存在的各种寿命组分提供产率分布的精确定位。这些结果表明，TD技术的生物医学成像与寿命敏感的有针对性的探针的潜力，并提供有用的指导方针的最佳方法，TD数据的荧光断层扫描。(c)2006年美国光学学会
A general linear model for time domain (TD) fluorescence tomography is presented that allows a lifetime-based analysis of the entire temporal fluorescence response from a turbid medium. Simulations are used to show that TD fluorescence tomography is optimally performed using two complementary approaches: A direct TD analysis of a few time points near the peak of the temporal response, which provides superior resolution; and an asymptotic multi-exponential analysis based tomography of the decay portion of the temporal response, which provides accurate localization of yield distributions for various lifetime components present in the imaging medium. These results indicate the potential of TD technology for biomedical imaging with lifetime sensitive targeted probes, and provide useful guidelines for an optimal approach to fluorescence tomography with TD data. (c) 2006 Optical Society of America