Estimating optical parameters of biological tissues with photon-counting micro-CT.
Estimating optical parameters of biological tissues with photon-counting micro-CT.
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DOI：
10.1364/josaa.451319
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发表时间：
2022-05-01
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生物组织的吸收和散射特性决定了荧光光子在生物组织中的传输和光学分子层析成像的分辨率。目前，这些参数是从光学测量数据计算的。对于小动物成像，光学参数的估计是一个大规模的优化问题，这是高度不适定的。在本文中，我们提出了一种新的，尽我们所知，光子计数微计算机断层扫描（micro-CT）的生物组织的光学参数估计的方法。根据光子计数X射线数据，可以重建多能量微型CT图像，以根据组织成分执行多器官分割和材料分解。主要组织成分的浓度和特性可以用于计算所涉及的组织的光学吸收和散射系数。在我们的研究中，我们进行了数值模拟，幻影实验，并在体内动物研究，使用我们提出的方法来计算光学参数。实验结果表明，该方法能够准确地估计生物组织的光学参数，相对误差小于10%，能够准确地绘制出生物组织的光学参数分布。
Wavelength-dependent absorption and scattering properties determine the fluorescence photon transport in biological tissues and image resolution of optical molecular tomography. Currently, these parameters are computed from optically measured data. For small animal imaging, estimation of optical parameters is a large-scale optimization problem, which is highly ill-posed. In this paper, we propose a new, to the best of our knowledge, approach to estimate optical parameters of biological tissues with photon-counting micro–computed tomography (micro-CT). From photon-counting x-ray data, multi-energy micro-CT images can be reconstructed to perform multi-organ segmentation and material decomposition in terms of tissue constituents. The concentration and characteristics of major tissue constituents can be utilized to calculate the optical absorption and scattering coefficients of the involved tissues. In our study, we perform numerical simulation, phantom experiments, and in vivo animal studies to calculate the optical parameters using our proposed approach. The results show that our approach can estimate optical parameters of tissues with a relative error of <10%, accurately mapping the optical parameter distributions in a small animal.