Evidence for a Role of Nitric Oxide in Hypovolemic Hemorrhagic Shock
Evidence for a Role of Nitric Oxide in Hypovolemic Hemorrhagic Shock
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一氧化氮在低血容量失血性休克中作用的证据
DOI：
10.1097/00005344-199206000-00021
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发表时间：
1992
影响因子：
3
通讯作者：
A. Caputi
中科院分区：
文献类型：
--
作者：
B. Zingarelli;F. Squadrito;D. Altavilla;G. Calapai;G. Campo;M. Caló;A. Saitta;A. Caputi
总结：通过从髂动脉导管间歇性抽血20 min，直至平均动脉压（MAP）降至30 mm Hg，诱导大鼠低血容量性失血性休克。然后评估存活率、存活时间、血浆心肌收缩因子（MAP）活性和胃的显微镜改变。L-精氨酸产生一氧化氮（NO）的选择性抑制剂NG-硝基-L-精氨酸甲酯（L-NAME）静脉注射（i. v.）在出血停止后。未治疗的失血性休克大鼠在27 ± 3.3 min内死亡，血浆TNF-α活性增强，胃底粘膜出现出血性浸润。L-NAME（5和10 mg/kg）可显著延长小鼠的存活率和存活时间，抑制血浆肌酐活性的升高，并对失血性低血容量性休克引起的胃损伤有保护作用。所有这些保护作用都被L-精氨酸（30 mg/kg/i. v.）的推注逆转，L-NAME给药后2 min给予。我们的研究结果表明，NO的产生在失血性休克的病理生理中起着重要的作用。
Summary: Hypovolemic hemorrhagic shock was induced in rats by intermittently withdrawing blood from an iliac catheter for 20 min until mean arterial blood pressure (MAP) decreased to 30 mm Hg. Survival rate, survival time, plasma myocardial depressant factor (MDF) activity, MAP, and microscopic gastric alterations were then evaluated. NG-nitro-L-arginine methyl-ester (L-NAME), a selective inhibitor of nitric oxide (NO) production from L-arginine, was injected intravenously (i.v.) after the bleeding was discontinued. Untreated hemorrhagic shocked rats died in 27 ± 3.3 min, had enhanced plasma activity of MDF, and exhibited hemorrhagic infiltrates in gastric fundus mucosa. L-NAME (5 and 10 mg/kg) significantly increased survival rate and time, blunted the increase in plasma MDF activity, and protected against the gastric lesions induced by hemorrhagic hypovolemic shock. All these protective effects were reversed by a bolus of L-arginine (30 mg/kg/i.v.), given 2 min after administration of L-NAME. Our findings suggest that NO production plays an important role in the pathophysiology of hemorrhagic shock.