Pan natural killer cell monoclonal antibodies and their relationship to the NK1.1 antigen.
Pan natural killer cell monoclonal antibodies and their relationship to the NK1.1 antigen.
复制标题
泛自然杀伤细胞单克隆抗体及其与 NK1.1 抗原的关系。
作者：
Sentman,CL;HackettJr,J;Moore,TA;Tutt,MM;Bennett,M;Kumar,V
自然杀伤（NK）的研究一直很困难，因为它们只占外周血和脾淋巴细胞的一小部分，而且已鉴定的 NK 特异性抗原很少。我们使用 IgG2b 单克隆抗体 PK136（抗 NK1.1）分离出纯 C57BL/6 (H-2b) NK 细胞群。这些 NK1.1+ 细胞用于免疫 129/J (H-2b) 小鼠，在本报告中，我们描述了三种新的 NK 特异性单克隆抗体（SW3A4(IgM)、SW4B12(IgG1) 和 SW2B4(IgG2b)）及其与已知的鼠 NK 抗原 NK1.1 的关系。我们进一步将 NK1.1 抗原定性为 39 kd 分子，该分子由似乎映射到 6 号染色体的基因编码。
The study of natural killer (NK) has been difficult because they account for a small percentage of peripheral blood and splenic lymphocytes and the paucity of NK specific antigens that have been identified. We have isolated pure populations of C57BL/6 (H-2b) NK cells using the IgG2b monoclonal antibody PK136 (anti-NK1.1). These NK1.1+cells were used to immunize 129/J (H-2b) mice, and in this report, we describe three new NK specific monoclonal antibodies (SW3A4(IgM), SW4B12(IgG1), and SW2B4(IgG2b)) and their relationship to the known murine NK antigen NK1.1. We have further characterized the NK1.1 antigen as a 39 kd molecule which is coded for by a gene which appears to map to chromosome 6.