Histological, cellular and behavioral assessments of stroke outcomes after photothrombosis-induced ischemia in adult mice.
Histological, cellular and behavioral assessments of stroke outcomes after photothrombosis-induced ischemia in adult mice.
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DOI：
10.1186/1471-2202-15-58
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发表时间：
2014-05-02
期刊：
影响因子：
2.4
通讯作者：
Ding S
中科院分区：
文献类型：
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作者：
Li H;Zhang N;Lin HY;Yu Y;Cai QY;Ma L;Ding S
在局灶性缺血性中风开始后，大脑经历了一系列改变，包括基础设施的演变，阴茎中的细胞增殖，而行为则定义了这些改变。
使用多种方法，我们通过测量脑损伤，动态细胞和神经胶质增殖来评估中风结果，以及在光疗（PT）诱导的成年小鼠中缺血性卒中后两周的不同时间进行的功能性缺陷。 ）标签的增殖细胞生成是时间上的空间依赖性，在缺血后第3-4天，该区域的增殖率最高，而在遥远的区域中，较高的速率是相似的时间依赖于时间的gfap+星形胶质细胞和iba1+微胶质细胞的次数。缺血性第2–4天。
我们的研究表明，首先是几天的关键时期，导致脑扩展，细胞增殖和行为定义在光栓塞引起的缺血模型中，而增殖的星形胶质细胞仅对增殖细胞的池和反应性星形胶质细胞只有很小的贡献。
Following the onset of focal ischemic stroke, the brain experiences a series of alterations including infarct evolvement, cellular proliferation in the penumbra, and behavioral deficits. However, systematic study on the temporal and spatial dependence of these alterations has not been provided. Using multiple approaches, we assessed stroke outcomes by measuring brain injury, dynamic cellular and glial proliferation, and functional deficits at different times up to two weeks after photothrombosis (PT)-induced ischemic stroke in adult mice. Results from magnetic resonance imaging (MRI) and Nissl staining showed a maximal infarction, and brain edema and swelling 1–3 days after PT. The rate of Bromodeoxyuridine (Brdu)-labeled proliferating cell generation is spatiotemporal dependent in the penumbra, with the highest rate in post ischemic days 3–4, and higher rate of proliferation in the region immediate to the ischemic core than in the distant region. Similar time-dependent generation of proliferating GFAP+ astrocytes and Iba1+ microglia/macrophage were observed in the penumbra. Using behavioral tests, we showed that PT resulted in the largest functional deficits during post ischemic days 2–4. Our study demonstrated that first a few days is a critical period that causes brain expansion, cellular proliferation and behavioral deficits in photothrombosis-induced ischemic model, and proliferating astrocytes only have a small contribution to the pools of proliferating cells and reactive astrocytes.
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影响因子：
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作者：
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通讯作者：
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