Global role of TATA box-binding protein recruitment to promoters in mediating gene expression profiles
Global role of TATA box-binding protein recruitment to promoters in mediating gene expression profiles
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DOI：
10.1128/mcb.24.18.8104-8112.2004
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发表时间：
2004-09-01
影响因子：
5.3
通讯作者：
Iyer, VR
中科院分区：
文献类型：
--
作者：
Kim, JW;Iyer, VR
TATA盒结合蛋白（TBP）的募集是转录起始过程中的限速步骤之一。然而，TBP募集在确定整个基因组转录的绝对和变化水平方面的全球重要性尚不清楚。我们使用基因组方法来探索TBP募集启动子和酿酒酵母中的全局基因表达谱之间的关系。我们的数据表明，首先，RNA聚合酶III启动子是TBP在体内最突出的结合靶点。第二，整个基因组中基因的稳态转录水平与TBP对其启动子的占用率成比例，这些基因表达水平的变化与TBP对其启动子的募集变化密切相关。第三，一个共识TATA元件似乎不是一个主要的决定因素，无论是TBP结合或基因表达在整个基因组。我们的研究结果表明，TBP在体内启动子的招聘是在确定酵母中的基因表达水平的普遍重要性，无论核心启动子的性质或类型的激活剂或阻遏物，可能介导的转录变化。此处报告的主要数据可在http://www.iyerlab.org/tbp上获取。
The recruitment of TATA box-binding protein (TBP) to promoters is one of the rate-limiting steps during transcription initiation. However, the global importance of TBP recruitment in determining the absolute and changing levels of transcription across the genome is not known. We used a genomic approach to explore the relationship between TBP recruitment to promoters and global gene expression profiles in Saccharomyces cerevisiae. Our data indicate that first, RNA polymerase III promoters are the most prominent binding targets of TBP in vivo. Second, the steady-state transcript levels of genes throughout the genome are proportional to the occupancy of their promoters by TBP, and changes in the expression levels of these genes are closely correlated with changes in TBP recruitment to their promoters. Third, a consensus TATA element does not appear to be a major determinant of either TBP binding or gene expression throughout the genome. Our results indicate that the recruitment of TBP to promoters in vivo is of universal importance in determining gene expression levels in yeast, regardless of the nature of the core promoter or the type of activator or repressor that may mediate changes in transcription. The primary data reported here are available at http://www.iyerlab.org/tbp.