Characterization of Echinostoma revolutum and Echinostoma robustum from ducks in Bangladesh based on morphology, nuclear ribosomal ITS2 and mitochondrial nad1 sequences
Characterization of Echinostoma revolutum and Echinostoma robustum from ducks in Bangladesh based on morphology, nuclear ribosomal ITS2 and mitochondrial nad1 sequences
复制标题
DOI：
10.1016/j.parint.2018.11.002
复制
发表时间：
2019-04-01
影响因子：
1.9
通讯作者：
Itagaki, Tadashi
中科院分区：
文献类型：
--
作者：
Mohanta, Uday Kumar;Watanabe, Takuya;Itagaki, Tadashi
精确区分棘口属物种内的'revolutum'组是相当困难的，因为它们的形态相似。本研究的目的是准确地描述棘口吸虫的孟加拉国鸭的形态和分子特征的基础上。鉴定出两种棘口吸虫：棘口吸虫E. revolutum和E.粗壮在系统发育树（ITS 2和nad 1）中，E. revolutum和E. robustum属于各自的欧亚林间空地，这是不同于美国林间空地。这些结果表明，这两个物种有两个不同的和地理上分离的谱系，欧亚和美洲。我们的分子和形态学数据结合先前发表的数据支持E. robustum、拟野牡丹E. miyagawai和E. Friedi先前基于分子或形态学证据。因此，这项研究提高了我们对“revolutum”组，特别是在亚洲的物种多样性的理解。
Precise discrimination of Echinostoma species within the 'revolutum' group is quite difficult because of their morphological similarities. The objective of this study was to precisely characterize the echinostomes of ducks from Bangladesh based on both morphological and molecular characteristics. Two Echinostoma species were identified: E. revolutum and E. robustum. In the phylogenetic trees (ITS2 and nad1), E. revolutum and E. robustum belonged to their respective Eurasian Glade, which is distinct from the American Glade. These results suggest that both species have two distinct and geographically separated lineages, Eurasian and American. Our molecular and morphological data combined with previously published data supports the synonymy of E. robustum, E. miyagawai, and E. friedi previously based on either molecular or morphological evidence. This study thus improves our understanding of species diversity of the 'revolutum' group, particularly in Asia.