Alterations in DNA methylation and genome structure in two rice mutant lines induced by high pressure
Alterations in DNA methylation and genome structure in two rice mutant lines induced by high pressure
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DOI：
10.1007/s11427-006-0097-3
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发表时间：
2006-03
期刊：
影响因子：
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通讯作者：
Sile Shen;Zhenwei Wang;X. Shan;Hua Wang;Lingdan Li;Xuyun Lin;L. Long;Kenan Weng;Bao Liu
中科院分区：
文献类型：
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作者：
Sile Shen;Zhenwei Wang;X. Shan;Hua Wang;Lingdan Li;Xuyun Lin;L. Long;Kenan Weng;Bao Liu
通过高压处理，从遗传稳定的野生稻品种北京38中获得了两个突变株系。用HpaII/MspI对突变系和原品系(北京38)的基因组DNA进行未消化或酶切，然后用ISSR和RAPD两个标记进行分子标记分析。结果表明，与原始水稻品种相比，两个突变品系的两个标记的扩增图谱均发生了明显的变化，表明突变品系中既有序列的改变，又有DNA甲基化的修饰。用两个细胞基因(S2和S3)、一组反转录转座子(Osr7、Osr36、Tos19等)和一个尘螨转座子家族(MPingandPong)进行的Southern杂交分析证实了这一结果，并表明在突变系中确实发生了DNA甲基化模式、基因组结构的变化，以及一些转座子可能的激活。此外，这些变化在自交的世代和不同的器官中保持稳定。因此，我们的结果表明，通过高压处理获得稳定的水稻突变体是可能的，突变体的分子基础可能包括遗传和表观遗传变化。因此，高静水压似乎是一种很有前途的植物诱变方法。
By using high-pressure treatment, two mutant lines were obtained from a genetically stablejaponicarice cultivar Bijing38. Genomic DNA of the mutant lines, together with the original line (Bijing38), was either undigested or digested byHpaII/MspI, and then subjected to molecular analysis using two markers, ISSR and RAPD. Results indicated that changes in the PCR amplification profiles of both markers are apparent in the two mutant lines compared with the original rice cultivar, suggesting that there had been both sequence changes and DNA methylation modifications in the mutant lines. Southern blot analysis using diverse sequences, including two cellular genes (S2 and S3), a set of retrotransposons (Osr7, Osr36, Tos19and more), and a MITE transposon family (mPingandPong), confirmed the results, and indicated that changes in DNA methylation pattern, genomic structure, and possible activation of some transposons indeed occurred in the mutant lines. Moreover, these changes are stably maintained through selfed generations and in different organs. Thus, our results indicate that it is possible to obtain stable mutants in rice by high pressure treatments, and the molecular basis of the mutants may include both genetic and epigenetic changes. Therefore, high hydrostatic pressure seems a promising approach for plant mutagenesis.