MORPHOMETRIC ANALYSIS OF CHONDROCYTE HYPERTROPHY
MORPHOMETRIC ANALYSIS OF CHONDROCYTE HYPERTROPHY
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DOI：
10.2106/00004623-198668020-00010
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发表时间：
1986-02-01
影响因子：
5.3
通讯作者：
GINSBERG, B
中科院分区：
文献类型：
--
作者：
BUCKWALTER, JA;MOWER, D;GINSBERG, B
在软骨生长板的肥大区，软骨细胞增大，呈现更球形的形状，并形成称为肥大软骨细胞的细胞群。参与肥大软骨细胞形成的机制知之甚少。细胞肥大通常是指与细胞器增加相关的细胞大小和体积的增加。在这项研究中，我们试图确定肥大软骨细胞的形成是否代表与细胞器增加相关的真正的细胞肥大，或者是否是由于肿胀和液体积累。对电子显微照片进行形态测定分析，以确定储备区、上增殖区、下增殖区、上肥大区和下肥大区中细胞数量、细胞体积、细胞器体积和基质体积的变化。在上增生区和下肥厚区之间，细胞的平均体积增加了500%.As他们扩大，他们的矩阵改变;领土矩阵体积增加，其胶原含量减少，和interterritorial矩阵体积减少，其胶原含量增加之间的下增生区和下肥厚区。之间的上部增殖区和下部肥大区，每个细胞的内质网，高尔基膜和线粒体的绝对体积增加了126%，而细胞质和核质的体积增加了779%，显然是由水的积累。下肥大区的细胞器未显示与细胞损伤或死亡相关的变化。因此，细胞器的合成有助于软骨细胞的扩大，但细胞扩大的主要机制是细胞质和核肿胀。
In the hypertrophic zone of the cartilaginous growth plate, chondrocytes enlarge, assume a more spherical shape, and form a population of cells called the hypertrophic chondrocytes. The mechanisms that are involved in the formation of hypertrophic chondrocytes are poorly understood. Cell hypertrophy usually refers to an increase in cell size and volume associated with an increase in organelles. In this study, we sought to determine whether the formation of hypertrophic chondrocytes represents true cell hypertrophy associated with an increase in organelles or whether it is due to swelling and fluid accumulation. Morphometric analyses of electron micrographs were carried out to determine changes in cell number, cell volume, cell organelle volumes, and matrix volumes in the reserve zone, upper proliferative zone, lower proliferative zone, upper hypertrophic zone, and lower hypertrophic zone. Between the upper proliferative zone and the lower hypertrophic zone, the cells increased their mean volume more than 500 per cent. As they enlarged, their matrices altered; territorial matrix volume increased as its collagen content decreased, and interterritorial matrix volume decreased as its collagen content increased between the lower proliferative zone and the lower hypertrophic zone. Between the upper proliferative zone and the lower hypertrophic zone, the absolute volume per cell of endoplasmic reticulum, Golgi membranes, and mitochondria increased 126 per cent, while the volume of cytoplasm and nucleoplasm increased 779 per cent, apparently by accumulation of water. Cell organelles of the lower hypertrophic zone did not show the changes that are associated with cell injury or death. Thus, the synthesis of organelles contributed to chondrocyte enlargement, but the primary mechanism of cell enlargement was cytoplasmic and nuclear swelling.