Baicalin, an inhibitor of HIV-1 production in vitro
Baicalin, an inhibitor of HIV-1 production in vitro
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DOI：
10.1016/s0166-3542(97)00069-7
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发表时间：
1998-02-01
影响因子：
7.6
通讯作者：
Tokunaga, T
中科院分区：
文献类型：
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作者：
Kitamura, K;Honda, M;Tokunaga, T
黄芩苷在植物血凝素（PHA）刺激的正常外周血单核细胞（PBMC）中以浓度依赖的方式显著抑制人类免疫缺陷病毒1型（HIV-1）的复制。用黄芩苷对细胞进行预处理，效果更明显。此外，黄芩苷抑制无症状HIV-1血清阳性携带者pha刺激的PBMC中HIV-1的复制。对HIV-1复制的50%抑制浓度约为0.5 μ g/ml。在黄芩苷浓度为2 μ g/ml时，HIV-1前病毒DNA的拷贝数比未处理的对照组少约50倍。在无细胞感染系统中，黄芩苷抑制HIV-1逆转录酶（RT）的活性，但不抑制人类DNA聚合酶α和γ的活性（DNA聚合酶β被轻微抑制），这表明黄芩苷的抗HIV-1作用可能至少部分是由于抑制HIV-1 RT。(C) 1998 Elsevier Science B.V.。
The flavonoid baicalin markedly inhibits replication of human immunodeficiency virus type 1 (HIV-1) in a concentration-dependent manner in normal peripheral blood mononuclear cells (PBMC) stimulated with phytohemagglutinin (PHA) in vitro. The effect was more pronounced when the cells were pretreated with baicalin. Furthermore, baicalin inhibits HIV-1 replication in PHA-stimulated PBMC from asymptomatic HIV-1-seropositive carriers. The 50% inhibitory concentration for HIV-1 replication was approximately 0.5 mu g/ml. At the concentration of 2 mu g/ml of baicalin, copy numbers of HIV-1 proviral DNA were approximately 50 times less than in untreated controls. In a cell-free infection system, baicalin inhibited the activity of HIV-1 reverse transcriptase (RT), but not the activity of human DNA polymerases alpha and gamma (DNA polymerase beta was slightly inhibited), suggesting that the anti-HIV-1 effect of baicalin may at least partly be due to inhibition of HIV-1 RT. (C) 1998 Elsevier Science B.V. All rights reserved.