Induction of matrix metalloproteinases and collagenolysis in chick embryonic membranes before hatching.
Induction of matrix metalloproteinases and collagenolysis in chick embryonic membranes before hatching.
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DOI：
10.1095/biolreprod60.1.183
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发表时间：
1999
影响因子：
3.6
通讯作者：
H. Lei;E. Furth;R. Kalluri;P. Wakenell;C. Kallen;J. Jeffrey;P. Leboy;J. Strauss
中科院分区：
文献类型：
--
作者：
H. Lei;E. Furth;R. Kalluri;P. Wakenell;C. Kallen;J. Jeffrey;P. Leboy;J. Strauss
鸡胚周围的膜在孵化前发生了显著的形态变化，其中包括尿囊膜的结构降解。胚期（ED） 20（孵化日）后，膜纤维胶原含量下降。到第19期，胚胎外液中出现了55-kDa的基质金属蛋白酶（MMP）活性，到第20期，胚胎膜提取物中出现了55-kDa的MMP活性。利用逆转录聚合酶链反应克隆了鸡的MMP-13 （55 ~ 57 kda）同源基因的部分cDNA。胚胎膜中MMP-13 mRNA的丰度在ED 19时显著增加，在ED 20时达到峰值。在胚胎外液中引入MMP抑制剂batimastat，可防止胚胎膜在孵化前发生结构变化。我们的结论是，像哺乳动物的胎膜一样，鸡胚膜在孵化前经历了末端重塑，部分原因是MMP活性的增加。鸡蛋系统代表了一种新的体内模型，用于探索出生前导致胚胎膜重塑的生化事件，并测试MMPs抑制剂防止胶原溶解和胎膜破裂的能力。
The membranes surrounding the chick embryo undergo striking morphological changes before hatching, which include structural degradation of the allantoic membrane. The fibrillar collagen content of the membranes declined by embryonic day (ED) 20 (the day of hatching). By ED 19, a 55-kDa matrix metalloproteinase (MMP) activity appeared in the extraembryonic fluid, and by ED 20 there was substantial 55-kDa MMP activity in embryonic membrane extracts. Reverse transcription-polymerase chain reaction was employed to clone a partial cDNA representing the chicken homologue of MMP-13, a 55- to 57-kDa enzyme. MMP-13 mRNA dramatically increased in abundance in embryonic membranes by ED 19, reaching a peak on ED 20. Introduction of the MMP inhibitor batimastat into the extraembryonic fluid prevented the structural changes in the embryonic membranes before hatching. We conclude that, like mammalian fetal membranes, chick embryonic membranes undergo terminal remodeling before hatching, in part as a result of increased MMP activity. The chicken egg system represents a novel in vivo model for exploring biochemical events leading to embryonic membrane remodeling prior to birth and to test inhibitors of MMPs for their ability to prevent collagenolysis and fetal membrane rupture.