HIGH MOLECULAR MASS FORMS OF BASIC FIBROBLAST GROWTH-FACTOR ARE INITIATED BY ALTERNATIVE CUG CODONS
HIGH MOLECULAR MASS FORMS OF BASIC FIBROBLAST GROWTH-FACTOR ARE INITIATED BY ALTERNATIVE CUG CODONS
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DOI：
10.1073/pnas.86.6.1836
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发表时间：
1989-03-01
影响因子：
11.1
通讯作者：
CAPUT, D
中科院分区：
文献类型：
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作者：
PRATS, H;KAGHAD, M;CAPUT, D
克隆并测序了 6.75 kb 的人肝癌来源的碱性成纤维细胞生长因子 (bFGF) cDNA。发现从纯化的肝癌bFGF产生的氨基末端序列对应于核苷酸序列，并且从推定的甲硫氨酸起始密码子上游8个氨基酸开始，被认为启动154个氨基酸的bFGF翻译产物。该序列表明存在至少 163 个氨基酸的 bFGF 形式。肝癌cDNA在体外转录成RNA； 该 RNA 的体外翻译产生了三种形式的 bFGF，分子量分别为 18、21 和 22.5 kDa。通过使用体外诱变，发现 22.5-kDa bFGF 和可能的 21-kDa 形式是用 CUG 起始密码子启动的。 18-kDa bFGF 由 AUG 密码子启动。通过将人肝癌bFGF cDNA和消除了AUG起始子的构建体转染至COS细胞中，发现bFGF的较高分子量形式与18-kDa形式具有相同的生物活性。
A 6.75-kilobase human hepatoma-derived basic fibroblast growth factor (bFGF) cDNA was cloned and sequenced. An amino-terminal sequence generated from a purified hepatoma bFGF was found to correspond to the nucleotide sequence and to begin 8 amino acids upstream from the putative methionine start condon thought to initiate a 154-amino acid bFGF translation product. This sequence suggests that a form of bFGF of at least 163 amino acid exists. The hepatoma cDNA was transcribed in vitro into RNA; in vitro translation of this RNA generated three forms of bFGF with molecular masses of 18, 21, and 22.5 kDa. By use of in vitro mutagenesis, it was found that the 22.5-kDa bFGF and possibly the 21-kDa form were initiated with CUG start condons. the 18-kDa bFGF was initiated with an AUG codon. By transfecting into COS cells human hepatoma bFGF cDNA and a construct from which the AUG initiator was eliminated, it was found that higher molecular mass forms by bFGF were as biologically active as the 18-kDa form.