Lymphocyte development from stem cells.
Lymphocyte development from stem cells.
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DOI：
10.1146/annurev.iy.10.040192.003551
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发表时间：
1992
影响因子：
29.7
通讯作者：
K. Ikuta;N. Uchida;J. Friedman;I. Weissman
中科院分区：
文献类型：
--
作者：
K. Ikuta;N. Uchida;J. Friedman;I. Weissman
从骨髓和胎儿肝脏中分离出高度富集的多能和专能造血干细胞 (HSC)，称为 Thy-1loLin-Sca-1+ 细胞。多能HSCs在其表面表达c-kit受体，但早期胎儿HSCs的产生和增殖是在没有钢因子的情况下发生的。 T前体细胞通过趋化机制迁移到胎儿胸腺中。 CD4lo前体代表了胸腺中骨髓源性干细胞和CD4-8-胸腺内前体之间新定义的T细胞发育阶段。只有胎儿而非成人的 HSC 具有在胎儿胸腺微环境下分化为 V gamma 3+ 和 V gamma 4+ T 细胞的能力，并且 HSC 本身可能在个体发育过程中失去部分发育潜力。据推测，HSC 是一个复杂但精确的发育时钟的位点，它可能决定一些基因位点（例如 V γ 3 和 V γ 4 T 细胞受体，以及胚胎和胎儿球蛋白）的时间依赖性闭合以及其他基因位点（例如 N 核苷酸插入机制）的激活。
Highly enriched pluripotent and multipotent hematopoietic stem cells (HSCs) are isolated from bone marrow and fetal liver as Thy-1loLin-Sca-1+ cells. Pluripotent HSCs express c-kit receptor on their surface, but the generation and proliferation of early fetal HSCs take place in the absence of steel factor. T precursor cells migrate into the fetal thymus by chemotactic mechanism. CD4lo precursors represent a newly defined phase of T-cell development in the thymus between the bone marrow-derived stem cells and the CD4-8- intrathymic precursors. Only fetal, but not adult, HSCs have the capacity to differentiate into V gamma 3+ and V gamma 4+ T cells under the fetal thymic microenvironment, and HSC themselves may lose some of their developmental potential during ontogeny. It is postulated that HSCs are the locus of a complicated but precise developmental clock that may determine both the time-dependent closure of some gene loci (e.g. V gamma 3 and V gamma 4 T cell receptor, and embryonic and fetal globin) and the activation of others (e.g. the N nucleotide insertion machinery).