Transient activation of Notch signaling in the injured adult brain
Transient activation of Notch signaling in the injured adult brain
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DOI：
10.1016/j.jchemneu.2009.09.003
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发表时间：
2010-01-01
影响因子：
2.8
通讯作者：
Wanaka, Akio
中科院分区：
文献类型：
--
作者：
Tatsumi, Kouko;Okuda, Hiroaki;Wanaka, Akio
脑损伤诱导各种细胞反应，导致组织再生和修复。最近的研究表明，常驻祖细胞增殖，然后分化为成熟的神经细胞。我们在这里发现，在低温损伤的大脑皮层中，增殖细胞在其细胞质中短暂表达Notch1免疫反应性。由于激活的Notch信号调节发育中的神经系统的细胞命运，因此在受伤的成人大脑中可能存在类似的调节。为了监测Notch信号通路，我们检测了信号通路的成分是否在notch1阳性细胞中共表达。早老素-1是一种跨膜蛋白酶，是Notch细胞内结构域释放所必需的，在Notch1阳性细胞和Hes1中被检测到，Hes1是Notch细胞内结构域的靶标，在低温损伤3天后也与Notch1共定位。这些结果表明Notch信号通路的瞬时活性参与了损伤脑中祖细胞增殖和分化的调控。(C) 2009 Elsevier B.V.版权所有
Brain injury induces various kinds of cellular responses that lead to tissue regeneration and repair. Recent studies have demonstrated that resident progenitors proliferate and then differentiate into mature neuronal cells. We show here that proliferating cells in the cryo-injured cerebral cortex transiently expressed Notch1 immunoreactivity in their cytoplasm. Since activated Notch signaling regulates cellular fate in the developing nervous system, similar regulation may exist in the injured adult brain. To monitor the Notch signaling pathway, we examined whether components of the signaling pathway were co-expressed in Notch1-positive cells. Presenilin-1, a membrane-spanning protease that is required for the release of the Notch intracellular domain, was detected in the Notch1-positive cells and Hes1, a target of the Notch intracellular domain, also co-localized with Notch1 three days after cryo-injury. These results suggest that transient activity of the Notch signaling pathway is involved in the regulation of proliferation and differentiation of progenitors in the injured brain. (C) 2009 Elsevier B.V. All rights reserved.