ADHESION FORCES BETWEEN INDIVIDUAL LIGAND-RECEPTOR PAIRS
ADHESION FORCES BETWEEN INDIVIDUAL LIGAND-RECEPTOR PAIRS
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DOI：
10.1126/science.8153628
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发表时间：
1994-04-15
期刊：
影响因子：
56.9
通讯作者：
GAUB, HE
中科院分区：
文献类型：
--
作者：
FLORIN, EL;MOY, VT;GAUB, HE
测量用抗生物素蛋白衍生的原子力显微镜悬臂尖端与用生物素、脱硫生物素或亚氨基生物素功能化的琼脂糖珠之间的粘附力。在仅允许有限数量的分子对相互作用的条件下，发现分离尖端和珠子所需的力被量化为生物素160+/-20皮牛顿和亚氨基生物素85+/-15皮牛顿的整数倍。测量的力量子被解释为各个分子对的解离力。
The adhesion force between the tip of an atomic force microscope cantilever derivatized with avidin and agarose beads functionalized with biotin, desthiobiotin, or iminobiotin was measured. Under conditions that allowed only a limited number of molecular pairs to interact, the force required to separate tip and bead was found to be quantized in integer multiples of 160 +/- 20 piconewtons for biotin and 85 +/- 15 piconewtons for iminobiotin. The measured force quanta are interpreted as the unbinding forces of individual molecular pairs.