Laser Treated Carbon Nanotube Yarn Microelectrodes for Rapid and Sensitive Detection of Dopamine in Vivo.
Laser Treated Carbon Nanotube Yarn Microelectrodes for Rapid and Sensitive Detection of Dopamine in Vivo.
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DOI：
10.1021/acssensors.6b00021
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发表时间：
2016-05-27
期刊：
影响因子：
8.9
通讯作者：
Venton BJ
中科院分区：
文献类型：
--
作者：
Yang C;Trikantzopoulos E;Nguyen MD;Jacobs CB;Wang Y;Mahjouri-Samani M;Ivanov IN;Venton BJ
碳纳米管纱线微电极(CNTYME)具有快速、选择性检测多巴胺的快速循环伏安法(FSCV)，但其灵敏度限制了其在体内的应用。在这项研究中，我们介绍了激光治疗作为一种简单、可靠和有效的方法，在保持高时间分辨率的同时将CNTYMES的灵敏度提高了三倍。用扫描电子显微镜、拉曼光谱、能量色散光谱和激光共聚焦显微镜表征了激光处理对微电极表面的影响。激光处理增加了比表面积和表面含氧官能团，这为多巴胺提供了比未修饰的CNTYME更多的吸附位置。此外，与未经修饰的CNTYME类似，激光处理CNTYME的多巴胺信号不依赖于扫描重复频率，不同于碳纤维微电极(CFME)的电流随着扫描重复频率的增加而减少。这种频率独立性是由于表面粗糙度大得多，这将捕获多巴胺邻苯二酚并放大多巴胺信号。首次将CNTYMES用作带有FSCV的活体传感器，与CFMES相比，经激光处理的CNTYMES保持了较高的多巴胺灵敏度，扫描重复频率增加了50赫兹，比传统频率快了五倍。利用激光处理的CNTYMEs具有制造简单、重复性高、电子传递动力学快、灵敏度高、体内快速测量多巴胺等优点，有望成为未来CFMEs的潜在替代品。
Carbon nanotube yarn microelectrodes (CNTYMEs) exhibit rapid and selective detection of dopamine with fast-scan cyclic voltammetry (FSCV); however, the sensitivity limits their application in vivo. In this study, we introduce laser treatment as a simple, reliable, and efficient approach to improve the sensitivity of CNTYMEs by three fold while maintaining high temporal resolution. The effect of laser treatment on the microelectrode surface was characterized by scanning electron microscopy, Raman spectroscopy, energy dispersion spectroscopy, and laser confocal microscopy. Laser treatment increases the surface area and oxygen containing functional groups on the surface, which provides more adsorption sites for dopamine than at unmodified CNTYMEs. Moreover, similar to unmodified CNTYMEs, the dopamine signal at laser treated CNTYMEs is not dependent on scan repetition frequency, unlike the current at carbon fiber microelectrodes (CFMEs) which decreases with increasing scan repetition frequency. This frequency independence is caused by the significantly larger surface roughness which would trap dopamine-o-quinone and amplify the dopamine signal. CNTYMEs were applied as an in vivo sensor with FSCV for the first time and laser treated CNTYMEs maintained high dopamine sensitivity compared to CFMEs with an increased scan repetition frequency of 50 Hz, which is five-fold faster than the conventional frequency. CNTYMEs with laser treatment are advantageous because of their easy fabrication, high reproducibility, fast electron transfer kinetics, high sensitivity, and rapid in vivo measurement of dopamine and could be a potential alternative to CFMEs in the future.