Texture of locomotor path: a replicable characterization of a complex behavioral phenotype
Texture of locomotor path: a replicable characterization of a complex behavioral phenotype
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DOI：
10.1111/j.1601-183x.2005.00126.x
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发表时间：
2005-10-01
影响因子：
2.5
通讯作者：
Elmer, GI
中科院分区：
文献类型：
--
作者：
Kafkafi, N;Elmer, GI
空间测试中小鼠运动路径的数据库可用于在计算机中搜索更好地区分基因型并且在实验室中更具可复制性的行为测量。在这项研究中，软件的探索探索（SEE）被用来搜索一个大型数据库的一种新的行为措施，将复杂的运动路径的特点。该数据库包括在3个实验室、7个近交系、几种药物治疗和数百只动物中评估的小鼠旷场行为。新的行为测量，“路径纹理”，其特征在于使用局部曲率的路径（每单位距离的方向变化，度/厘米）在几个空间尺度，从规模小于动物的身体长度和规模的竞技场大小。路径纹理分析与分维分析的不同之处在于它不假设跨尺度的自相似性。路径纹理被发现区分自交系具有相对高的广义遗传力（43%-71%）和高复制性在实验室之间。即使在某些尺度上具有相似路径曲率的基因型在其他尺度上也通常不同，并且并非所有基因型都显示出跨尺度的自相似性。安非他明可降低C57 BL/6小鼠的路径曲率，但对DBA/2 J小鼠无影响。地西泮密切依赖性地降低C57 BL/6小鼠在所有尺度上的曲率，而2种致焦虑药物FG-7142和戊四唑增加了它。因此，路径纹理在小鼠的行为表型和药物作用的研究中具有很高的潜力。
A database of mouse locomotor path in spatial tests can be used to search in silico for behavioral measures that better discriminate between genotypes and are more replicable across laboratories. In this study, software for the exploration of exploration (SEE) was used to search a large database for a novel behavioral measure that would characterize complex movement paths. The database included mouse open-field behavior assessed in 3 laboratories, 7 inbred strains, several pharmacological treatments and hundreds of animals. The new behavioral measure, 'path texture', was characterized using the local curvature of the path (the change of direction per unit distance, in degrees/cm) across several spatial scales, starting from scales smaller than the animal's body length and up to the scale of the arena size. Path texture analysis differs from fractal dimension analysis in that it does not assume self-similarity across scales. Path texture was found to discriminate inbred strains with relatively high broad-sense heritability (43%-71%) and high replicability across laboratories. Even genotypes that had similar path curvatures in some scales usually differed in other scales, and self-similarity across scales was not displayed by all genotypes. Amphetamine decreased the path curvature of C57BL/6 mice in small and medium scales, while having no effect on DBA/2J mice. Diazepam close-dependently decreased the curvature of C57BL/6 mice across all scales, while 2 anxiogenic drugs, FG-7142 and pentylenetetrazole, increased it. Path texture thus has high potential for behavioral phenotyping and the study of drug effects in the mouse.