Characterization of Curli A Production on Living Bacterial Surfaces by Scanning Probe Microscopy
Characterization of Curli A Production on Living Bacterial Surfaces by Scanning Probe Microscopy
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DOI：
10.1016/j.bpj.2012.09.004
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发表时间：
2012-10-17
影响因子：
3.4
通讯作者：
Park, Sungsu
中科院分区：
文献类型：
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作者：
Oh, Yoo Jin;Cui, Yidan;Park, Sungsu
卷曲是由许多肠杆菌科（Enterobacteriaceae）产生的粘附性表面纤维，例如大肠杆菌（Escherichia coli）和沙门氏菌（Salmonella enterica）。它们与细菌附着和侵入上皮细胞有关。本研究利用原子力显微镜研究了卷曲对活大肠杆菌的拓扑结构和力学性能的影响。coli细胞。卷曲缺陷和卷曲过度生产突变体的杨氏模量显着低于其野生型（WT）菌株，而前者的菌株的衰变长度高于后者的菌株。令人惊讶的是，拓扑图像显示，与WT和卷曲过度产生突变体不同，卷曲缺陷突变体产生了大量的鞭毛样纤维，这可以解释为什么该菌株具有比WT更低的杨氏模量。这些结果表明，细菌表面的机械性能的存在下，如卷曲和鞭毛丝状结构的影响很大。
Curli are adhesive surface fibers produced by many Enterobacteriaceae, such as Escherichia coli and Salmonella enterica. They are implicated in bacterial attachment and invasion to epithelial cells. In this study, atomic force microscopy was used to determine the effects of curli on topology and mechanical properties of live E. coli cells. Young's moduli of both curli-deficient and curli-overproducing mutants were significantly lower than that of their wild-type (WT) strain, while decay lengths of the former strains were higher than that of the latter strain. Surprisingly, topological images showed that, unlike the WT and curli-overproducing mutant, the curli-deficient mutant produced a large number of flagella-like fibers, which may explain why the strain had a lower Young's modulus than the WT. These results suggest that the mechanical properties of bacterial surfaces are greatly affected by the presence of filamentous structures such as curli and flagella.