RHEUMATOID FACTORS AND SECONDARY IMMUNE-RESPONSES IN THE MOUSE .2. INCIDENCE, KINETICS AND INDUCTION MECHANISMS
RHEUMATOID FACTORS AND SECONDARY IMMUNE-RESPONSES IN THE MOUSE .2. INCIDENCE, KINETICS AND INDUCTION MECHANISMS
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DOI：
10.1002/eji.1830131107
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发表时间：
1983-01-01
影响因子：
5.4
通讯作者：
VANSNICK, J
中科院分区：
文献类型：
--
作者：
COULIE, P;VANSNICK, J
通过测量脾细胞培养物中类风湿因子（RF）的合成，研究了正常小鼠品系在二次免疫应答期间IgM RF的产生。在BALB/c和C57 BL/6小鼠中观察到相当大的RF反应，尽管是短暂的，但用各种蛋白抗原和绵羊红细胞免疫和静脉内加强。对于大多数抗原，RF的产生等于或超过抗原特异性IgM的产生，但其达到峰值并较早消退。当省略初免或加强时，未检测到RF产生。RF的同种型特异性取决于用于免疫的抗原：所有测试的蛋白质刺激合成RF特异性IgG 1，而绵羊红细胞诱导IgG 1和IgG 2a特异性RF。用抗人转铁蛋白的同基因抗体和引发或未引发的脾细胞的各种组合对辐照的幼稚C57 BL/6小鼠进行重建实验，表明只有当抗原注射到已经接受抗体和免疫脾细胞的小鼠中时才能诱导重要的RF应答。
Production of IgM rheumatoid factor (RF) during secondary immune responses was studied in normal mouse strains by measuring RF synthesis in spleen cell cultures. A considerable, although transient, RF response was observed in BALB/c and C57BL/6 mice immunized and i.v. boosted with various protein antigens and with sheep erythrocytes. With most antigens, RF production equaled or exceeded that of antigen-specific IgM, but it peaked and subsided earlier. No RF production was detected when either priming or boosting was omitted. The isotypic specificity of RF depended upon the antigens used for immunization: all protens tested stimulated the synthesis of RF specific for IgG1, whereas sheep erythrocytes induced both IgG1- and IgG2a-specific RF. Reconstitution experiments of irradiated naive C57BL/6 mice with various combinations of syngeneic antibody against human transferrin and primed or unprimed spleen cells indicated that important RF responses could be induced only when antigen was injected into mice that had received both antibody and immune spleen cells.