Lipidic vector systems for gene transfer.
Lipidic vector systems for gene transfer.
复制标题
用于基因转移的脂质载体系统。
DOI：
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发表时间：
1997
影响因子：
2.7
通讯作者：
Leaf Huang
中科院分区：
文献类型：
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作者：
Robert J. Lee;Leaf Huang
基因治疗的临床应用有赖于开发合适的基因转移载体(载体)。尽管通常不如病毒载体那么有效，但非病毒系统，如脂类载体，具有毒性小、对DNA大小无限制、潜在的靶向性好、易于相对大量生产等潜在优势。更重要的是，脂类载体通常缺乏免疫原性，允许使用相同的载体重复体内转染。在这篇文章中，我们将试图总结一些最新的研究进展，在脂质基因传递载体。介绍了三种脂类基因转移载体：1)DNA/阳离子脂质体复合体，2)中性或阴离子脂质体包裹的DNA，以及3)脂质体包裹的聚阳离子缩合DNA(LPDI和LPDII)。我们综述了影响载体结构和基因传递效率的各种因素，并讨论了基因转移的可能机制及其在载体设计中的意义。
Clinical application of gene therapy depends on the development of suitable gene transfer vehicles (vectors). Although generally not as efficient as viral vectors, nonviral systems such as lipidic vectors have the potential advantages of being less toxic, nonrestrictive in cargo DNA size, potentially targetable, and easy to produce in relatively large amounts. More important, lipidic vectors generally lack immunogenicity, allowing repeated in vivo transfection using the same vector. In this paper, we will attempt to summarize some of the recent advances in lipidic gene delivery vectors. Three types of lipidic gene transfer vectors are described: 1) DNA/cationic liposome complexes, 2) DNA encapsulated in neutral or anionic liposomes, and 3) liposome-entrapped, polycation-condensed DNA (LPDI and LPDII). We review the various factors affecting vector structure and gene delivery efficiency, and we discuss the possible mechanisms of gene transfer and their implications in vector design.