CHARACTERIZATION OF A HAIR (WOOL) KERATIN INTERMEDIATE FILAMENT GENE DOMAIN
CHARACTERIZATION OF A HAIR (WOOL) KERATIN INTERMEDIATE FILAMENT GENE DOMAIN
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DOI：
10.1111/1523-1747.ep12371758
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发表时间：
1994-02-01
影响因子：
6.5
通讯作者：
BELTRAME, JS
中科院分区：
文献类型：
--
作者：
POWELL, BC;BELTRAME, JS
在上皮分化过程中，角蛋白中间丝基因以多种组织特异性和阶段特异性模式表达。属于多基因家族的一对I型和II型中间丝基因协同调节，每种类型的4-5个基因在毛囊中表达。积累的染色体作图数据指向每个中间丝多基因家族在不同染色体上的一个主要基因座。在这份报告中，我们描述了一个绵毛角蛋白粘粒的分离和染色体行走，该粘粒与前面描述的两个粘粒重叠，并建立了一个连续的100kb的克隆DNA片段，其中包含三个毛发和三个毛状II型中间细丝角蛋白基因。一个新的毛发角蛋白II型中间丝基因KRT2.11位于簇的中间，部分序列数据显示其预测的N端区域与其他绵毛角蛋白II型中间丝蛋白具有显著的保守性。表达分析表明，在羊毛毛囊RNA中存在2.4kb的KRT2.11转录本，并且在毛乳头上方的毛囊皮质角质形成细胞中表达。这三个毛发基因聚集在约40kb的范围内，两侧是毛发类基因，这些基因在毛囊中不表达，从而划分了毛发角蛋白基因结构域。
In epithelial differentiation keratin intermediate filament genes are expressed in multifarious tissue-specific and stage-specific patterns. Pairs of type I and type II intermediate filament genes, belonging to multigene families, are coordinately regulated, and 4 - 5 genes of each type are expressed in the hair follicle. Accumulating chromosomal mapping data points to a major locus for each intermediate filament multigene family on separate chromosomes. In this report we describe the isolation of a sheep hair keratin cosmid and chromosome walking that overlaps two previously described cosmids and establishes a continuous 100-kb segment of cloned DNA containing three hair and three hair-like type II intermediate filament keratin genes. A new hair keratin type II interme-diate filament gene, KRT2.11, is located in the middle of the cluster, and partial sequence data reveal a striking conservation of its predicted N-terminal region with other sheep hair keratin type II intermediate filament proteins. Expression analyses demonstrate the presence of a 2.4-kb KRT2.11 transcript in wool follicle RNA and show that expression occurs in the follicle cortical keratinocytes above the dermal papilla. The three hair genes are clustered within about 40 kb and flanked by hair-like genes that are not expressed in the hair follicle, thereby demarcating a hair keratin gene domain.