New Insights into the Neural Differentiation Potential of Canine Adipose Tissue-Derived Mesenchymal Stem Cells
New Insights into the Neural Differentiation Potential of Canine Adipose Tissue-Derived Mesenchymal Stem Cells
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DOI：
10.1111/ahe.12270
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发表时间：
2017-06-01
影响因子：
0.9
通讯作者：
Arnhold, S.
中科院分区：
文献类型：
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作者：
Blecker, D.;Elashry, M. I.;Arnhold, S.
脂肪组织来源的干细胞（ASC）可以从体内不同的脂肪组织来源获得。它是一个丰富的细胞库，容易获得，适合体外培养和扩增，并为治疗方法做准备。这些治疗方法包括组织工程和神经系统疾病，如脊髓损伤。对于这种治疗，ASC必须可靠地分化为神经元方向。因此，我们研究了神经分化潜能的ASCs使用协议与神经诱导剂，如丙戊酸和毛喉素，而狗脑组织作为对照。神经元诱导后1h即可观察到形态学变化。基因表达分析显示，神经元标记物巢蛋白和III-微管蛋白以及MAP 2的神经元分化诱导后表达。另外，测定神经营养因子NGF、BDNF和GDNF的表达。一些神经元标记物和神经营养因子在未分化细胞中已经表达。我们的研究结果指出，ASC可以可靠地分化为神经元谱系;因此，这些细胞是用于中枢神经系统疾病的细胞移植的合适细胞来源。后续研究将显示这些细胞移植后的临床益处。
Adipose tissue-derived stem cells (ASCs) can be obtained from different adipose tissue sources within the body. It is an abundant cell pool, easily accessible, suitable for cultivation and expansion invitro and preparation for therapeutic approaches. Amongst these therapeutic approaches are tissue engineering and nervous system disorders such as spinal cord injuries. For such treatment, ASCs have to be reliably differentiated in to the neuronal direction. Therefore, we investigated the neural differentiation potential of ASCs using protocols with neurogenic inductors such as valproic acid and forskolin, while dog brain tissue served as control. Morphological changes could already be noticed 1h after neuronal induction. Gene expression analysis revealed that the neuronal markers nestin and III-tubulin as well as MAP2 were expressed after induction of neuronal differentiation. Additionally, the expression of the neurotrophic factors NGF, BDNF and GDNF was determined. Some of the neuronal markers and neurotrophic factors were already expressed in undifferentiated cells. Our findings point out that ASCs can reliably be differentiated into the neuronal lineage; therefore, these cells are a suitable cell source for cell transplantation in disorders of the central nervous system. Follow-up studies would show the clinical benefit of these cells after transplantation.