Examining Site-Specific GPCR Phosphorylation
Examining Site-Specific GPCR Phosphorylation
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DOI：
10.1007/978-1-61779-126-0_12
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Kong, Kok Choi
中科院分区：
文献类型：
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作者：
Butcher, Adrian J.;Tobin, Andrew B.;Kong, Kok Choi
G 蛋白偶联受体 (GPCR) 的磷酸化是激动剂刺激介导的最重要的翻译后修饰之一。该过程已被证明不仅导致受体脱敏，而且还通过招募抑制蛋白接头蛋白，促进受体与多种信号传导途径偶联。此外，现在越来越多的证据表明，GPCR 可能利用磷酸化作为调节其细胞类型特异性信号传导的机制，从而产生组织特异性功能。这些进步部分归功于确定 GPCR 上磷酸受体位点的方法的改进以及对磷酸化后果的分析的改进。本章旨在描述我们实验室用于研究 M-3-毒蕈碱受体位点特异性磷酸化的方法。这些方法可以很容易地应用于其他受体的研究。
Phosphorylation of G protein-coupled receptors (GPCRs) is one of the most prominent post-translation modifications mediated by agonist stimulation. This process has been shown to result not only in receptor desensitisation but also, via the recruitment of arrestin adaptor proteins, to promote receptor coupling to numerous signalling pathways. Furthermore, there is now a growing body of evidence suggesting that GPCRs may employ phosphorylation as a mechanism to regulate their cell-type-specific signalling, hence generating tissue-specific functions. These advances have resulted partly from improved methods used in the determination of phospho-acceptor sites on GPCRs and improved analysis of the consequences of phosphorylation. This chapter aims to describe the methods used in our laboratory for the investigation of site-specific phosphorylation of the M-3-muscarinic receptor. These methods could easily be applied in the study of other receptors.