Failure of alleles at the Na+, K(+)-ATPase alpha 1 locus to cosegregate with blood pressure in Dahl rats.
Failure of alleles at the Na+, K(+)-ATPase alpha 1 locus to cosegregate with blood pressure in Dahl rats.
复制标题
Dahl 大鼠中 Na , K( )-ATPase α 1 位点的等位基因未能与血压共分离。
DOI：
10.1097/00004872-199005000-00010
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发表时间：
1990
影响因子：
4.9
通讯作者：
Dene,H
中科院分区：
文献类型：
--
作者：
Rapp,JP;Dene,H
测试先前描述的Dahl大鼠中Na+、K+-腺苷5'-三磷酸酶(ATP酶)α1亚型基因座处的pst I限制性片段长度多态性，以检测分离群体中与血压的共分离。将近交系 Dahl 盐敏感 (S) 和近交系 Dahl 耐盐 (R) 大鼠杂交产生 F1 大鼠，并产生三个分离群体：F1 x R、F2 和 F1 x S，并在不同时期内以高盐饮食饲养，以获得每个群体的最大血压反应。在无隔离群体中，Na+、K+-ATP酶α1基因座的基因型之间的血压不存在显着差异。功效计算表明，< 7.5 mmHg 的单等位基因剂量效应不太可能被检测到。结论是，在这些实验条件下（高饮食盐、Dahl大鼠遗传背景），Na+、K+-ATP酶α1基因座处的S和R等位基因不影响血压，或者即使影响，其影响也很小。
A previously described pst I restriction fragment length polymorphism in Dahl rats at the Na+, K+-adenosine 5'-triphosphatase (ATPase)[alpha] 1 isoform locus was tested for cosegregation with blood pressure in segregating populations. Inbred Dahl salt-sensitive (S) and inbred Dahl salt-resistant (R) rats were crossed to produce F1 rats, and three segregating populations, F1 x R, F2 and F1 x S, were produced and raised on a high-salt diet for variable periods to attain the maximal blood pressure response in each population. In no segregating population was there a significant difference in blood pressure among genotypes at the Na+, K+-ATPase [alpha] 1 locus. Power calculations suggest that single-allele dosage effects of< 7.5 mmHg were unlikely to be detected. It is concluded that either the S and R alleles at the Na+, K+-ATPase [alpha] 1 locus do not influence blood pressure under the conditions of these experiments (high dietary salt, Dahl rat genetic background) or, if they do, their effect is small.