Studies of pollen maturation in cotton: the storage reserve accumulation phase
Studies of pollen maturation in cotton: the storage reserve accumulation phase
复制标题
棉花花粉成熟研究：储存储备积累阶段
作者：
C. L. R. Wetzel;W. Jensen
本研究以棉花（Gossypium hirsutum L.）花粉粒为材料，特别是营养细胞质的发育和形成的各种贮藏产物。CTEM、HVEM、体视学和细胞组织化学用于检查开花前9天期间发生的事件。淀粉在开花前9天开始在质体中积累，并在开花前不久达到峰值浓度;脂质沉积遵循类似的模式，但在开花前6天开始。脂质体常被紧密压迫在内质网上。在整个9天期间，网质体表现活跃;首先产生参与内壁形成的小泡，然后产生储存在花粉粒中的小泡。网体囊泡似乎含有多糖，并集中在脂质体周围的层中。核糖体的数量从开花前6天开始增加，并且在成熟花粉中特别多。从开花期到开花期的6天内，内质网池变得越来越膨胀，在花粉释放前的几个小时内，形成充满脂质体和网状体小泡的口袋。成熟花粉的核心区域充满ER口袋，而外周细胞质中通常缺乏这种口袋。
SummaryThis study follows the maturation of the pollen grain of cotton (Gossypium hirsutum L.), particularly the development of the vegetative cytoplasm and the various storage products formed. CTEM, HVEM, stereoscopy, and cyto-histochemistry were used to examine the events occurring during the 9 days before anthesis. Starch began to accumulate in plastids at anthesis minus 9 days and reached a peak concentration shortly before anthesis; lipid deposition followed a similar pattern, but started at 6 days before anthesis. Lipid bodies were always seen closely oppressed to the endoplasmic reticulum (ER). Dictyosomes appear active during the entire 9 days; first producing vesicles involved in the formation of the intine and, later, producing vesicles stored in the pollen grain. The dictyosome vesicles appear to contain polysaccharides and concentrate in layers around the lipid bodies. Ribosomes increase in number from 6 days before anthesis and are particularly numerous in the mature pollen. From anthesis minus 6 days until anthesis, the ER cisternae become increasingly inflated and, in the hours immediately before pollen release, form pockets filled with lipid bodies and dictysosome vesicles. The mature pollen has a core region filled with ER pockets and a peripheral cytoplasm in which such pockets are generally lacking.