2 HUMAN 35 KD INHIBITORS OF PHOSPHOLIPASE-A2 ARE RELATED TO SUBSTRATES OF PP60V-SRC AND OF THE EPIDERMAL GROWTH-FACTOR RECEPTOR-KINASE
2 HUMAN 35 KD INHIBITORS OF PHOSPHOLIPASE-A2 ARE RELATED TO SUBSTRATES OF PP60V-SRC AND OF THE EPIDERMAL GROWTH-FACTOR RECEPTOR-KINASE
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DOI：
10.1016/0092-8674(86)90736-1
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发表时间：
1986-07-18
期刊：
影响因子：
64.5
通讯作者：
PEPINSKY, RB
中科院分区：
文献类型：
--
作者：
HUANG, KS;WALLNER, BP;PEPINSKY, RB
我们已经从人胎盘中纯化了两个35 kd的磷脂酶A2抑制剂，我们称之为脂皮质素I和II。这两种蛋白质表现出相似的生物化学性质，在胎盘中约占总蛋白质的0.2%。通过肽图谱，序列和免疫学分析，我们表明，脂皮质素I和35 kd的EGF受体/激酶从A431细胞的底物是相同的蛋白质。通过类似的标准，我们确定，脂皮质素II是人类的类似物的pp 36，一个主要的底物pp 60 src，其特征在于在鸡胚成纤维细胞和牛刷状缘制剂。我们从cDNA克隆中推导出的脂皮质素I和II的氨基酸序列有50%的同源性，表明它们可能是从一个共同的基因进化而来的。
We have purified two 35 kd phospholipase A2 inhibitors from human placenta, which we refer to as lipocortin I and II. Both proteins exhibit similar biochemical properties and occur in placenta at about 0.2% of the total protein. By peptide mapping, sequence, and immunological analyses, we show that lipocortin I and the 35 kd substrate for the EGF-receptor/kinase from A431 cells are the same protein. By similar criteria, we determine that lipocortin II is the human analogue of pp36, a major substrate for pp60src, which has been characterized in chicken embryo fibroblasts and in bovine brush border preparations. The amino acid sequences of lipocortin I and II that we deduced from cDNA clones share 50% homology, indicating that they probably evolved from a common gene.