miR-30c and semaphorin 3A determine adult neurogenesis by regulating proliferation and differentiation of stem cells in the subventricular zones of mouse
miR-30c and semaphorin 3A determine adult neurogenesis by regulating proliferation and differentiation of stem cells in the subventricular zones of mouse
复制标题
miR-30c和semaphorin 3A通过调节小鼠脑室下区干细胞的增殖和分化来决定成体神经发生
DOI：
10.1111/cpr.12261
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发表时间：
2016-06-01
影响因子：
8.5
通讯作者：
Ling, Shucai
中科院分区：
文献类型：
--
作者：
Sun, Tingting;Li, Weiyun;Ling, Shucai
目的成体神经干细胞增殖的调控机制尚不清楚。在这里，我们研究了microR-30 c（miR-30 c）和它的目标，信号3A（sema 3A），在调节成人神经发生和机制的作用，这一进程的基础上，Materials and MethodsIn原位杂交，免疫荧光和定量实时PCR被用来评估小鼠的miR-30 c和sema 3A的互补表达模式。通过立体定位注射上调和下调慢病毒来检查室管膜下区（SVZ）中miR-30 c的作用。进行5-溴-2-脱氧尿苷标记以研究miR-30 c和sema 3A对成体神经发生的影响。实时细胞分析，形态学分析和细胞周期测量被用来揭示miR-30 c和sema 3A调节成人neurogenes. ResultsmiR-30 c的表达与sema 3A在神经元中的负相关，和miR-30 c和sema 3A的水平与SVZ和吻侧迁移流中的新生细胞的数量呈正相关。结论miR-30 c和sema 3A通过调控SVZ干细胞的增殖和分化来调控成体神经发生。这一发现揭示了一种新的成人神经发生的调节机制。
ObjectivesMechanisms that regulate proliferation of adult neural stem cells are largely unknown. Here, we have investigated the role of microR-30c (miR-30c) and its target, semaphoring 3A (sema3A), in regulating adult neurogenesis and mechanisms underlying this process.Materials and methodsIn situ hybridization, immunofluorescence and quantitative real-time PCR were used to assess complementary expression patterns of miR-30c and sema3A in mice. Effects of miR-30c in the subventricular zone (SVZ) were examined by stereotaxic injection of up- and down-regulating lentiviruses. 5-bromo-2-deoxyuridine labelling was performed to investigate effects of miR-30c and sema3A on adult neurogenesis. Real-time cell assays, morphological analysis and cell cycle measurements were used to reveal the mechanisms by which miR-30c and sema3A regulate adult neurogenesis.ResultsExpression of miR-30c negatively correlated with that of sema3A in neurons, and levels of miR-30c and sema3A correlated positively with numbers of newborn cells in the SVZ and rostral migration stream. miR-30c and sema3A affected adult neurogenesis by regulating proliferation and differentiation, as well as cycles of stem cells in the SVZ.ConclusionsmiR-30c and sema3A regulate adult neurogenesis by controlling proliferation and differentiation of stem cells in the SVZ. This finding reveals a novel regulatory mechanism of adult neurogenesis.